LOCUS       sequence-114224-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 4-6 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX2-6
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(387..404)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(387..403)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(396..418)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      467..553
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      645..672
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgtcacccgg caaccttggg tagcagcgaa gtcgaggcat ttctgtcctg gctggtctag
       61 acgtctcgcc atggtcctgg cacagcaccg gcaacagccg ctgcaccgtt tcgagcgctt
      121 gcttgccgcc atcgtggctg gcgatagcca ccacttggtc cggagtcagg ccatggtcct
      181 ggcacagcac cggcaacagc cgctgcaccg tttcgagcgc ttgcttgccg ccatcgtggc
      241 tggcgatagc caccacttgg tccggagtca ggccatggtc ctggcacagc accggcaaca
      301 gccgctgcac cgtttcgagc gcttgcttgc cgccaatgtt gctggcgata gccaccactt
      361 ggtccggggt cagggagacg cttaagactg gccgtcgttt tacaacgtcg tgactgggaa
      421 aacatccatg ctagcgttaa cgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
      481 ggctcagtcg gaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
      541 gagtaggaca aatccgccgg gagcggattt gaacgttgtg aagcaacggc ccggagggtg
      601 gcgggcagga cgcccgccat aaactgccag gcatcaaact aagcagaagg ccatcctgac
      661 ggatggcctt tttgcgtttc tacaaactct tcctggcta
//
