LOCUS       sequence-114234-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector expressing human TRF1 delta aa317-374 with 
            N-terminal MYC tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Zimmermann M, Kibe T, Kabir S, de Lange T
  TITLE     TRF1 negotiates TTAGGG repeat-associated replication problems by 
            recruiting the BLM helicase and the TPP1/POT1 repressor of ATR 
            signaling.
  JOURNAL   Genes Dev. 2014 Nov 15;28(22):2477-91. doi: 10.1101/gad.251611.114. 
            Epub 2014 Oct 24.
  PUBMED    25344324
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.251611.114"; journalName: "Genes Dev"; date: 
            "2014-11-15- 15"; volume: "28"; issue: "22"; pages: "2477-91"
COMMENT     Sequence Label: pLPC-NMYC-hTRF1deltaBLM
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             86..115
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttgg
       61 taccgagctc ggatcagcca ccatggaaca aaagttgatt tctgaagaag atttgttaag
      121 aaagagagga tccgcggagg atgtttcctc agcggccccg agcccgcggg gctgtgcgga
      181 tggtagggat gccgacccta ctgaggagca gatggcagaa acagagagaa acgacgagga
      241 gcagttcgaa tgccaggaac tgctcgagtg ccaggtgcag gtgggggccc cagaggagga
      301 ggaggaggag gaggaggacg cgggcctggt ggccgaggcc gaggccgtgg ctgccggctg
      361 gatgctcgat ttcctctgcc tctctctttg ccgagctttc cgcgacggcc gctccgagga
      421 cttccgcagg acccgcaaca gcgcagaggc tattattcat ggactatcca gtctaacagc
      481 ttgccagttg agaacgatat acatatgtca gtttttgaca agaattgcag caggaaaaac
      541 ccttgatgca cagtttgaaa atgatgaacg aattacaccc ttggaatcag ccctgatgat
      601 ttggggttca attgaaaagg aacatgacaa acttcatgaa gaaatacaga atttaattaa
      661 aattcaggct atagctgttt gtatggaaaa tggcaacttt aaagaagcag aagaagtctt
      721 tgaaagaata tttggtgatc caaattctca tatgcctttc aaaagcaaat tgcttatgat
      781 aatctctcag aaagatacat ttcattcctt ttttcaacac ttcagctaca accacatgat
      841 ggagaaaa
//
