LOCUS       sequence-114251-        2121 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of fluorescent membranes in sea urchin primordial germ 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Campanale JP, Gokirmak T, Espinoza JA, Oulhen N, Wessel GM, Hamdoun 
            A
  TITLE     Migration of sea urchin primordial germ cells.
  JOURNAL   Dev Dyn. 2014 Jul;243(7):917-27. doi: 10.1002/dvdy.24133. Epub 2014 
            Apr 30.
  PUBMED    24677545
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.24133"; journalName: "Dev Dyn"; date: "2014-07"; 
            volume: "243"; issue: "7"; pages: "917-27"
COMMENT     Sequence Label: CmCitrine Lck w/ NosUTRs
FEATURES             Location/Qualifiers
     source          1..2121
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      214..223
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      277..286
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(283..285,286..288,289..801)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(328..349)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(589..608)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     937..958
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cacgtgcctc attaaagccc acataaatga gtttgttgca catgtggata aattgcaata
       61 ctttctcaaa ttcaatttag atcattcttg actagctcta cgacgtacta cgctctttac
      121 aaaatttact gtctcgagtt tccacgcact ttagtcaccg gatccatggg ctgcatcaag
      181 agcaagcgca aggacaacct gaacgacgac gaggccgcca tgggttgtat taaatcaaaa
      241 cgaaaagata atttgaatga tgatgaagca ccggtcgcca ccatggtgag caagggcgag
      301 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      361 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      421 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc
      481 tacggcctga tgtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
      541 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      601 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      661 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      721 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
      781 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      841 acccccatcg gcgacnggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
      901 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      961 cgccgccggg atcactctcg gcatggacga gctgtacaag taaactagtt ctgagcagcc
     1021 tttgcagcac gacacttcct tggctcagct ccgttcttcc aactttgcca gcgaacgaca
     1081 accgactatg gacataaacg actttttatt tggacctgtg cagttagttt gatgtagggt
     1141 ctactcgtct cgaggttgat gagacttgga caactcattt tttaatttct ttttcttgcc
     1201 agtggatttt actgaacagt tcgaactgac tgaattctta agtgtaacta actaaattca
     1261 tgtggattgt gttcacagca gggtggttta ttataagttt tctgcttgtg cagttgtgta
     1321 tgtttttttt gtacaggtgc ttcgcgtatg tattcaaatt tcatacctcg agaggctcaa
     1381 gtttgatttt aatcgtttgt ccatgattgg atgtaaccac agtaaatgtt attgtttata
     1441 aatcgacatg tcattacttc gtattaactg ttaatgtatt ctgatgtttt tgctatatac
     1501 atgaggatct cggaagtaga atttcaagtc tccaattttt tacattaatg caggttattc
     1561 actgcttcgc gatgggagtt ttcttgtgga aattcattgc gagaagtatt ttttaaagta
     1621 ggttattata tatttaaaat gcaatttcaa ttattcattt atttgaactt tttgtattat
     1681 gtatatttta agaccaattt gtcaatatat cgtgctaatt atgtaatgat tgtgattaaa
     1741 cggacctaag tgaaaattgt gattttaaga tgtacaaaca tttctcattt tgatgaaggt
     1801 tgtaatagag gtatcttggc acagcatcat tagttttatc agatagaagt ctgacgttaa
     1861 gaaaattgat tactgccttt acaactagca ggcacattgc tttcttaact ctgaatacat
     1921 gaatatacat aatattgtat aagtaaagca cattcgttac ctgtaaagtc attttgttta
     1981 catgagataa ttggttttag tgtttatcga gaataaccac atttatgcaa ttcttaatgt
     2041 catttatgtt aaatgaaatc agaaagtact gattgctggg tgtattaatt aaatgtacga
     2101 aggtactaca ttcttttaaa a
//
