LOCUS       sequence-114254-        9624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of murine PDGFRalpha tagged with GFP under the control of
            EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9624)
  AUTHORS   Nielsen BS, Malinda RR, Schmid FM, Pedersen SF, Christensen ST, 
            Pedersen LB
  TITLE     PDGFRbeta and oncogenic mutant PDGFRalpha D842V promote disassembly 
            of primary cilia through a PLCgamma- and AURKA-dependent mechanism.
  JOURNAL   J Cell Sci. 2015 Oct 1;128(19):3543-9. doi: 10.1242/jcs.173559. Epub
            2015 Aug 19.
  PUBMED    26290382
REFERENCE   2  (bases 1 to 9624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.173559"; journalName: "J Cell Sci"; date: "2015-10-1- 
            1"; volume: "128"; issue: "19"; pages: "3543-9"
COMMENT     Sequence Label: pcDNA5FRT-EF-Pdgfralpha-EGFPN
FEATURES             Location/Qualifiers
     source          1..9624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        247..1429
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          478..1420
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1377..1397
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1844..1855
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      4805..4814
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4811..4813,4814..4816,4817..5530)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4811..4813,4814..4816,4817..5527)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4856..4877)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5117..5136)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5464..5485
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5576..5593)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5582..5806
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5939..5958)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6090..6137
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6145..7167
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6145..7167
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7297..7418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7334..7353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7388..7407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7467..7483)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7467..7483)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7480..7502)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7491..7507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7515..7521,7522..7539,7540..7545))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7560..7581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7698..7715)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7869..8457)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7949..7968)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8628..9419,9420..9488))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9251..9270
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9489..9593)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtaggagtg
      241 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
      301 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
      361 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
      421 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
      481 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
      541 tgaattactt ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
      601 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
      661 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
      721 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
      781 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
      841 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
      901 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
      961 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     1021 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     1081 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     1141 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     1201 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     1261 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     1321 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     1381 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattctcta
     1441 gagatccctc gacctcgaga tccattgtgc tggatctaca atggccccgc tagcgtttaa
     1501 acttaagctt atggggacct cccaccaggt ctttctggtc ctcagctgtc tcctcacagg
     1561 gccgggcctc atctcctgcc agctcttatt accctctatc ctcccaaacg agaatgagaa
     1621 gattgtgcag ctgaattcgt ccttttctct gagatgcgtt ggggagagtg aagtgagctg
     1681 gcagcacccc atgtctgaag aagacgaccc caacgtggaa atcagaagtg aggagaacaa
     1741 cagtggcctc tttgtcacag tgctggaagt ggttaacgcc tccgcagccc acaccggatg
     1801 gtacacttgc tactacaacc acactcagac ggatgagagt gagatcgaag gcaggcacat
     1861 ttacatctat gtaccagacc cagacatggc ctttgtgcct ctcgggatga ccgattcctt
     1921 agtcatcgtg gaagaggatg actctgccat cataccttgc cgcaccacag atccggagac
     1981 tcaagtaacc ttgcacaata acgggaggct ggtgcctgcc tcctatgaca gcaggcaggg
     2041 cttcaacgga accttcagcg tggggcctta catctgtgag gccaccgtca aagggaggac
     2101 gttcaagacc agcgagttta atgtttatgc cttgaaagca acgtcagaac tgaatctgga
     2161 gatggacgca cgccagactg tgtataaggc aggagaaacg atcgtggtga cctgtgccgt
     2221 ctttaacaac gaggtcgttg acctgcagtg gacttaccct ggagaagtga gaaacaaagg
     2281 tatcaccatg ctggaggaga tcaaactccc atccatcaaa ctggtgtaca ctttgaccgt
     2341 ccccaaggcc acggtgaagg acagtggaga gtatgaatgt gctgcccgtc aggccactaa
     2401 agaggtcaag gaaatgaaga gagtcaccat ttctgtccac gagaaaggct tcgtcgagat
     2461 tgagcccacc tttggccagc tggaagctgt caacttgcac gaagtcagag agttcgtggt
     2521 ggaggtgcag gcctacccga cgcccaggat atcgtggctg aaggacaact tgactctgat
     2581 agagaatctc accgagatca ccactgatgt acagaagagt caggaaacaa ggtatcagag
     2641 caaattaaag ctgatccggg ctaaggaaga agacagtggc cattacacca ttatagttca
     2701 aaatgaggat gacgtgaaga gttacacgtt tgagctgtca accctagttc ctgcatccat
     2761 tttggatctc gtagatgacc accatggctc tggcggggga cagactgtga ggtgtacagc
     2821 cgaaggcacc cctcttccag aaatcgactg gatgatctgc aagcatatta agaaatgtaa
     2881 taatgacact tcgtggacag ttttggccag caatgtctca aatattatca cagagctccc
     2941 tcgccgtggc aggagtaccg tggagggacg agtgtccttc gccaaagtgg aagagaccat
     3001 cgcagttcga tgcctggcaa agaacaacct cagcgttgtg gcccgtgagc tgaagctggt
     3061 ggctcccact ctgcgatctg aactcacagt ggcggccgca gtgttggtgc tgttggtgat
     3121 tgtcattgtc tctctcatcg tcctggtggt catttggaag cagaaaccac ggtatgaaat
     3181 tcgctggagg gttatcgagt caatcagccc cgatggacat gagtatatct atgtggaccc
     3241 catgcagttg ccttacgact ccagatggga attcccgaga gatggcctcg tgcttggtcg
     3301 gattttggga tccggtgcat ttgggaaagt ggtcgaaggt acagcttatg gattaagccg
     3361 gtcccaacct gtaatgaagg tggctgtgaa gatgctcaaa cccacagcca ggtctagtga
     3421 aaagcaggct ctcatgtctg agctgaagat aatgactcac ctggggccac atttgaacat
     3481 tgtgaacttg ctgggagcct gcaccaagtc aggtcccatt tacatcatca cagaatactg
     3541 cttctatggg gatttggtca actacttgca taagaacaga gatagcttca tgagccaaca
     3601 cccagagaag ccgaagaaag acctggacat ctttggattg aatcctgcag acgagagcac
     3661 aagaagttat gtgattttgt cttttgaaaa taacggcgac tacatggaca tgaagcaagc
     3721 tgataccaca cagtatgtcc ccatgcttga aaggaaagag gtttctaaat actctgacat
     3781 ccagagatcg ctgtacgatc ggccagcctc ctacaagaag aaatccatgc tagactcaga
     3841 agtcaaaaac ctcctttcgg acgatgactc cgagggtctg actttgctgg atctattgag
     3901 cttcacctat caagttgctc gaggaatgga atttttggct tcgaaaaatt gtgtccaccg
     3961 ggacctggct gcccgcaacg tcctcctggc gcaaggaaaa attgtgaaga tctgtgattt
     4021 tggcctggcc agagacatca tgcacgattc caactacgtg tcaaaaggca gtactttcct
     4081 gcctgtgaag tggatggcac ccgagagcat cttcgacaac ctctacacca cgctgagtga
     4141 cgtctggtcc tatggcattc tgctctggga aatcttttcc cttggtggca caccctatcc
     4201 tggcatgatg gtcgattcta ctttctacaa taagatcaag agtggatacc ggatggccaa
     4261 acctgaccat gccaccagtg aagtctatga gatcatggtg cagtgctgga acagtgagcc
     4321 cgagaagaga ccctccttct accacctcag cgagatagtg gagaacctgt tgccgggaca
     4381 atacaagaag agttatgaaa agattcacct ggacttccta aagagtgacc atccggccgt
     4441 ggcacgcatg cgggtggact ctgataatgc gtacatcggt gtcacttaca aaaatgaaga
     4501 ggataagctg aaggactggg aaggtggcct ggacgaacag agactgagcg ctgacagtgg
     4561 ctacatcatc cccctgccag acattgaccc tgttccagag gaggaagacc tgggcaagag
     4621 gaacagacac agctcacaga cttcggaaga gagtgccatc gagacaggtt ccagtagttc
     4681 caccttcatc aagagagagg acgagaccat cgaggacatc gacatgatgg atgacattgg
     4741 tatagattcc tcggacctgg tggaggacag cttcctggta ccgcgggccc gggatccacc
     4801 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     4861 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     4921 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     4981 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     5041 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     5101 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     5161 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     5221 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     5281 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     5341 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     5401 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     5461 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     5521 gtacaagtaa agcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     5581 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5641 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5701 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5761 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     5821 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     5881 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5941 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6001 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6061 cttgattagg gtgatggttc acgtacctag aagttcctat tccgaagttc ctattctcta
     6121 gaaagtatag gaacttcctt ggccaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     6181 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     6241 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     6301 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     6361 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
     6421 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     6481 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     6541 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     6601 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     6661 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     6721 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     6781 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     6841 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     6901 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     6961 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     7021 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     7081 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     7141 agcactcgtc cgagggcaaa ggaatagcac gtactacgag atttcgattc caccgccgcc
     7201 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     7261 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     7321 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7381 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     7441 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7501 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7561 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7621 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7681 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7741 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7801 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7861 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7921 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7981 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8041 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8101 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8161 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8221 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8281 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     8341 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8401 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8461 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8521 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8581 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8641 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8701 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8761 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8821 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8881 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8941 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9001 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9061 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9121 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9181 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9241 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9301 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9361 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9421 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9481 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9541 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9601 ccccgaaaag tgccacctga cgtc
//
