LOCUS       sequence-114256-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 1-3 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX1-54
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(398..415)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(398..414)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(407..429)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      478..564
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      656..683
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tcacccggca accttgggta gcagcgaagt cgaggcattt ctgtcctggc tggtctagac
       61 gtctcgtcag gccatggtcc tggcacagca ccggcaacag ccgctgcacc gtttcgagcg
      121 cttgcttgcc gccatcgtgg ctggcgatag ccaccacttg gtccggagtc aggccatggt
      181 cctggcacag caccggcaac agccgctgca ccgtttcgag cgcttgcttg ccgccatcgt
      241 ggctggcgat agccaccact tggtccggag tcaggccatg gtcctggcac agcaccggca
      301 acagccgctg caccgtttcg agcgcttgct tgccgccacc gttgctggcg atagccacca
      361 cttggtccgg ggtcaggttc agggggagac gcttaagact ggccgtcgtt ttacaacgtc
      421 gtgactggga aaacatccat gctagcgtta acgcgagagt agggaactgc caggcatcaa
      481 ataaaacgaa aggctcagtc ggaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      541 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgt gaagcaacgg
      601 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaac taagcagaag
      661 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttcctggcta gcggtacgcg
      721 tattaattgc g
//
