LOCUS       sequence-114261-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 1-3 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX1-23
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(399..416)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(399..415)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(408..430)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      479..565
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      657..684
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtcacccggc aaccttgggt agcagcgaag tcgaggcatt tctgtcctgg ctggtctaga
       61 cgtctcgtca ggccatggtc ctggcacagc accggcaaca gccgctgcac cgtttcgagc
      121 gcttgcttgc cgccattgtt gctggcgata gccaccactt ggtccggggt caggccatgg
      181 tcctggcaca gcaccggcaa cagccgctgc accgtttcga gcgcttgctt gccgccatcg
      241 tggctggcga tagccaccac ttggtccgga gtcaggccat ggtcctggca cagcaccggc
      301 aacagccgct gcaccgtttc gagcgcttgc ttgccgccat cgtggctggc gatagccacc
      361 acttggtccg gggtcaggtt cagggggaga cgcttaagac tggccgtcgt tttacaacgt
      421 cgtgactggg aaaacatcca tgctagcgtt aacgcgagag tagggaactg ccaggcatca
      481 aataaaacga aaggctcagt cggaagactg ggcctttcgt tttatctgtt gtttgtcggt
      541 gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg tgaagcaacg
      601 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa
      661 ggccatcctg acggatggcc tttttgcgtt tctacaaact cttcctggct agcggta
//
