LOCUS       sequence-114295-         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 4-6 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX2-31
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(390..407)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(390..406)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(399..421)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      470..556
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      648..675
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtgcgtcacc cggcaacctt gggtagcagc gaagtcgagg catttctgtc ctggctggtc
       61 tagacgtctc gccatggtcc tggcacagca ccggcaacag ccgctgcacc gtttcgagcg
      121 cttgcttgcc gccattgttg ctggcgatag ccaccacttg gtccggggtc aggccatggt
      181 cctggcacag caccggcaac agccgctgca ccgtttcgag cgcttgcttg ccgccaccgt
      241 tgctggcgat agccaccact tggtccgggg tcaggccatg gtcctggcac agcaccggca
      301 acagccgctg caccgtttcg agcgcttgct tgccgccatc gtggctggcg atagccacca
      361 cttggtccgg ggtcagggag acgcttaaga ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaacatcc atgctagcgt taacgcgaga gtagggaact gccaggcatc aaataaaacg
      481 aaaggctcag tcggaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      541 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gtgaagcaac ggcccggagg
      601 gtggcgggca ggacgcccgc cataaactgc caggcatcaa actaagcaga aggccatcct
      661 gacggatggc ctttttgcgt ttctacaaac tcttcctg
//
