LOCUS       sequence-114302-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 1-3 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX1-56
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(401..418)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(401..417)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(410..432)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      481..567
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      659..686
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcgtcacccg gcaaccttgg gtagcagcga agtcgaggca tttctgtcct ggctggtcta
       61 gacgtctcgt caggccatgg tcctggcaca gcaccggcaa cagccgctgc accgtttcga
      121 gcgcttgctt gccgccaccg ttgctggcga tagccaccac ttggtccggg gtcaggccat
      181 ggtcctggca cagcaccggc aacagccgct gcaccgtttc gagcgcttgc ttgccgccat
      241 cgtggctggc gatagccacc acttggtccg gagtcaggcc atggtcctgg cacagcaccg
      301 gcaacagccg ctgcaccgtt tcgagcgctt gcttgccgcc accgttgctg gcgatagcca
      361 ccacttggtc cggggtcagg ttcaggggga gacgcttaag actggccgtc gttttacaac
      421 gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag agtagggaac tgccaggcat
      481 caaataaaac gaaaggctca gtcggaagac tgggcctttc gttttatctg ttgtttgtcg
      541 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgtgaagcaa
      601 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aactaagcag
      661 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttc
//
