LOCUS       sequence-11431-e        1554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Machacek M, Hodgson L, Welch C, Elliott H, Pertz O, Nalbant P, Abell
            A, Johnson GL, Hahn KM, Danuser G
  TITLE     Coordination of Rho GTPase activities during cell protrusion.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):99-103.
  PUBMED    19693013
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):99-103."
COMMENT     Sequence Label: pBabe-Ypet-PBD
FEATURES             Location/Qualifiers
     source          1..1554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..28
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(433..452)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    1159..1175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(1243..1266)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
       61 ccatagaaga caccgggacc gatccagcct ccgcggcccc agatccgtaa agtgaattct
      121 agcaccatgg tgagcaaggg cgaagagctg ttcaccggcg tggtgcccat cctggtggag
      181 ctggacggcg acgtgaacgg ccacaagttc agcgtgagcg gcgagggcga gggcgacgcc
      241 acctacggca agctgaccct gaagctgctg tgcaccaccg gcaagctgcc cgtgccctgg
      301 cccaccctgg tgaccaccct gggctacggc gtgcagtgct tcgcccggta ccccgaccac
      361 atgaagcagc acgacttctt caagagcgcc atgcccgagg gctacgtgca ggagcggacc
      421 atcttcttca aggacgacgg caactacaag acccgggccg aggtgaagtt cgagggcgac
      481 accctggtga accggatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      541 ggccacaagc tggagtacaa ctacaacagc cacaacgtgt acatcaccgc cgacaagcag
      601 aagaacggca tcaaggccaa cttcaagatc cggcacaaca tcgaggacgg cggcgtgcag
      661 ctggccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      721 aaccactacc tgagctacca gagcgccctg ttcaaggacc ccaacgagaa gcgggaccac
      781 atggtgctgc tggagttcct gaccgccgcc ggcatcaccg agggcatgga cgagctgtac
      841 aagtccggac tcagatctga gctcggatcc attttacctg gagataaaac aaataaaaag
      901 aaagagaaag agcggccaga gatttctctc ccttcagatt ttgaacacac aattcatgtc
      961 ggttttgatg ctgtcacagg ggagtttacg ggaatgccag agcagtgggc ccgcttgctt
     1021 cagacatcaa atatcactaa gtcggagcag aagaaaaacc cgcaggctgt tctggatgtg
     1081 ttggagtttt acaactcgaa gaagacatcc aacagccaga aatgaattaa ttcgttaacg
     1141 catgcctcga gtgtggaatt gtgagcggat aacaatttgt cgagggcccc tgcaggtcaa
     1201 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     1261 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     1321 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     1381 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     1441 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaaagcgggt
     1501 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctca
//
