LOCUS       sequence-114322-         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) 
            [E:LacZa:G].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: DVA_EG
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    9..30
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(25..348)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCRRHKLRP
                     TSL"
     primer_bind     243..265
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     257..274
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     258..274
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    275..331
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(344..360)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(344..360)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(357..379)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    368..384
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(392..398,399..416,417..422))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    437..458
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    558..578
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(584..601)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gatttctgga attcgcggcc gcttctagag actagtgggt ctcagcttat gtcttctgca
       61 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
      121 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      181 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      241 ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta cccggggatc
      301 ctctagagtc gacctgcagg catgcaagct tggcgtaatc atggtcatag ctgtttcctg
      361 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      421 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      481 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      541 agacgttgcc agagacctac tagtagcggc cgctgcaggc ttcctcgctc actgactcgc
      601 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      661 tatccacaga atcaggg
//
