LOCUS       sequence-114338-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector expressing mouse TRF1 with N-terminal Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Zimmermann M, Kibe T, Kabir S, de Lange T
  TITLE     TRF1 negotiates TTAGGG repeat-associated replication problems by 
            recruiting the BLM helicase and the TPP1/POT1 repressor of ATR 
            signaling.
  JOURNAL   Genes Dev. 2014 Nov 15;28(22):2477-91. doi: 10.1101/gad.251611.114. 
            Epub 2014 Oct 24.
  PUBMED    25344324
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.251611.114"; journalName: "Genes Dev"; date: 
            "2014-11-15- 15"; volume: "28"; issue: "22"; pages: "2477-91"
COMMENT     Sequence Label: pLPC-NMYC-mTRF1
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      76..85
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             85..114
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagcttggt
       61 accgagctcg gatcagccac catggaacaa aagttgattt ctgaagaaga tttgttaaga
      121 aagagaggat ccgcggagac ggtctcctca gcggcccggg acgcgccgag ccgtgagggc
      181 tggacagatt cggattctcc agagcaggag gaggtgggag acgacgcgga gctgctccag
      241 tgccagcttc agctggggac cccgagagag atggagaacg cggagcttgt ggctgaggtg
      301 gaggccgtgg ctgcgggctg gatgctcgac ttcctctgcc tgtctctgtg ccgagccttc
      361 cgcgacggcc gctccgagga ctttcgtcgt actcgtgaca gcgccgaggc tattattcat
      421 ggactacaca gacttacagc ttaccaattg aaaactgtgt atatatgtca gtttttgaca
      481 agagttgcat ctggaaaggc ccttgatgca cagtttgaag ttgatgagcg tattacaccc
      541 ttggaatcag ccctgatgat ttggaactca attgaaaagg aacatgacaa actgcatgac
      601 gaaataaaga atttaattaa aattcaggct gtagctgttt gtatggaaat tggcagcttt
      661 aaggaagcag aagaagtatt tgaaagaata tttggtgatc cagaatttta cacgccttta
      721 gaaaggaagt tacttaagat aatctctcag aaggatgtgt tccactccct tttccaacac
      781 ttcagctata gctgcatgat ggagaaaatt cagagttatg tgggtgatgt gttaagtgaa
      841 aaatcatcaa cttttctaat gaaggcagca acaaaagtag tggaaaatga gaaagcgagg
      901 acacaagcgt ctaaggatag gccagatgcc accaacactg gaatggacac tgaagttggt
      961 ttgaataaag agaaaagtgt taatggcca
//
