LOCUS       sequence-114351-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..690
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagtgtgat taaaccagag
       61 atgaagatga ggtactacat ggacggctcc gtcaatgggc atgagttcac aattgaaggt
      121 gaaggcacag gcagacctta cgagggacat caagagatga cactacgcgt cacaatggcc
      181 gagggcgggc caatgccttt cgcgtttgac ttagtgtcac acgtgttctg ttacggccac
      241 agagtattta ctaaatatcc agaagagata ccagactatt tcaaacaagc atttcctgaa
      301 ggcctgtcat gggaaaggtc gttggagttc gaagatggtg ggtccgcttc agtcagtgcg
      361 catataagcc ttagaggaaa caccttctac cacaaatcca aatttactgg ggttaacttt
      421 cctgccgatg gtcctatcat gcaaaaccaa agtgttgatt gggagccatc aaccgagaaa
      481 attactgcca gcgacggagt tctgaagggt gatgttacga tgtacctaaa acttgaagga
      541 ggcggcaatc acaaatgcca aatgaagact acttacaagg cggcaaaaga gattcttgaa
      601 atgccaggag accattacat cggccatcgc ctcgtcagga aaaccgaagg caacattact
      661 gagcaggtag aagatgcagt agctcatagc tccggactca gatctggcag cggtggaggc
      721 agcgcatccg gcggaagcgg aagcgatgat gatatcgccg cgctcgtcgt cgacaacggc
//
