LOCUS       sequence-114353-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi Complex, Excitation: 485, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfGFP-Golgi-7
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      283..292
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(289..291,292..294,295..807)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVE"
     primer_bind     complement(334..355)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgaaccgtca gatccgctag catgaggctt cgggagccgc tcctgagcgg cagcgccgcg
       61 atgccaggcg cgtccctaca gcgggcctgc cgcctgctcg tggccgtctg cgctctgcac
      121 cttggcgtca ccctcgttta ctacctggct ggccgcgacc tgagccgcct gccccaactg
      181 gtcggagtct ccacaccgct gcagggcggc tcgaacagtg ccgccgccat cgggcagtcc
      241 tccggggagc tccggaccgg aggggccaag gatccaccgg tcgccaccat ggtgagcaag
      301 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      361 ggccacaagt tcagcgtgcg cggcgagggc gagggcgatg ccaccaacgg caagctgacc
      421 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      481 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagcg ccacgacttc
      541 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcagctt caaggacgac
      601 ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      661 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      721 aacttcaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
      781 aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc agctcg
//
