LOCUS       sequence-114355-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: Dronpa-Mito-7
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..88
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      116..125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             122..799
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 cagatccgct agcatgtccg tcctgacgcc gctgctgctg cggggcttga caggctcggc
       61 ccggcggctc ccagtgccgc gcgccaagat ccattcgttg ggggatccac cggtcgccac
      121 catggtgagt gtgattaaac cagacatgaa gatcaagctg cgtatggaag gcgctgtaaa
      181 tggacacccg ttcgcgattg aaggagttgg ccttgggaag cctttcgagg gaaaacagag
      241 tatggacctt aaagtcaaag aaggcggacc tctgcctttc gcctatgaca tcttgacaac
      301 tgtgttctgt tacggcaaca gggtattcgc caaataccca gaaaatatag tagactattt
      361 caagcagtcg tttcctgagg gctactcttg ggaacgaagc atgaattacg aagacggggg
      421 catttgtaac gcgacaaacg acataaccct ggatggtgac tgttatatct atgaaattcg
      481 atttgatggt gtgaactttc ctgccaatgg tccagttatg cagaagagga ctgtgaaatg
      541 ggagccatcc actgagaaat tgtatgtgcg tgatggagtg ctgaagggtg atgttaacat
      601 ggctctgtcg cttgaaggag gtggccatta ccgatgtgac ttcaaaacta cttataaagc
      661 taagaaggtt gtccagttgc cagactatca ctttgtggac caccacattg agattaaaag
      721 ccacgacaaa gattacagta atgttaatct gcatgagcac gccgaagcgc attctgagct
      781 gccgaggcag gccaagtaat gataggcggc cgcgactcta gatcataatc agccatacca
      841 catttgtaga ggttttactt gctttaaaaa acctccnaca cctccccctg aacctgaaac
      901 ataaaatgaa tgcaattgtt
//
