LOCUS       sequence-11437-e        8791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   Edwards SR, Wandless TJ
  TITLE     Dicistronic regulation of fluorescent proteins in the budding yeast 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2010 Apr;27(4):229-36. doi: 10.1002/yea.1744
  PUBMED    20017217
REFERENCE   2  (bases 1 to 8791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea"; 
            journalName: "Yeast"; date: "2010-04"; volume: "27"; issue: "4"; 
            pages: "229-36"
COMMENT     Sequence Label: pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR
FEATURES             Location/Qualifiers
     source          1..8791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..719
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1633..2346
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1633..2346
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2356..2832
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDHVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     terminator      3219..3406
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3274..3298)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(3523..4617)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4618..5023)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(5065..5081)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5065..5081)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5078..5100)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5089..5105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5113..5119,5120..5137,5138..5143))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5158..5179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    5234..5267
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5345..5378
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5447..5464)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5618..6206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5698..5717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6377..7168,7169..7237))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7000..7019
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7238..7342)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7410..7428
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7466..7488)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7588..7607
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      7624..8788
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggatcctt ttgttgtttc cgggtgtaca atatggactt cctcttttct
       61 ggcaaccaaa cccatacatc gggattccta taataccttc gttggtctcc ctaacatgta
      121 ggtggcggag gggagatata caatagaaca gataccagac aagacataat gggctaaaca
      181 agactacacc aattacactg cctcattgat ggtggtacat aacgaactaa tactgtagcc
      241 ctagacttga tagccatcat catatcgaag tttcactacc ctttttccat ttgccatcta
      301 ttgaagtaat aataggcgca tgcaacttct tttctttttt tttcttttct ctctcccccg
      361 ttgttgtctc accatatccg caatgacaaa aaaaatgatg gaagacacta aaggaaaaaa
      421 ttaacgacaa agacagcacc aacagatgtc gttgttccag agctgatgag gggtatctcg
      481 aagcacacga aactttttcc ttccttcatt cacgcacact actctctaat gagcaacggt
      541 atacggcctt ccttccagtt acttgaattt gaaataaaaa aagtttgctg tcttgctatc
      601 aagtataaat agacctgcaa ttattaatct tttgtttcct cgtcattgtt ctcgttccct
      661 ttcttccttg tttctttttc tgcacaatat ttcaagctat accaagcata caatcaactc
      721 cggtaccact agtatggtaa gtaagggtga agaagacaat atggcgatca ttaaggaatt
      781 catgcgtttc aaagtacaca tggagggaag cgtgaacgga catgaatttg aaatcgaagg
      841 ggaaggcgaa ggtagaccat acgaaggaac ccagaccgca aagcttaaag ttaccaaagg
      901 cgggccacta ccatttgcat gggatatctt gagccctcag tttatgtatg gcagtaaggc
      961 ctacgttaaa cacccagctg atattcccga ctatttgaaa ttgtcttttc cagaaggatt
     1021 caaatgggaa agagtaatga atttcgagga cggcggagtt gttactgtta ctcaagattc
     1081 aagtttgcaa gacggtgaat ttatttacaa ggtcaaatta agagggacta atttccctag
     1141 tgatggtccc gtcatgcaaa agaagactat gggttgggaa gcctcatctg aacgtatgta
     1201 tccagaagat ggcgcgctta agggggaaat taaacaaaga ttgaagttaa aagacggtgg
     1261 tcactacgac gcggaagtta agaccactta taaagctaaa aagcccgttc agttacctgg
     1321 tgcatataac gtaaacatta aattggatat cacttcacat aatgaagatt acactattgt
     1381 ggaacaatat gaaagagctg aaggtaggca ctcaacgggt ggaatggacg aattgtacaa
     1441 ataaccgccg cgtttaccga ttaagcacag tacctttacg ttatatatag gattggtgtt
     1501 tagctttttt tcctgagccc ctggttgact tgtgcatgaa cacgagccat ttttagtttg
     1561 tttaagggaa gttttttgcc acccaaaacg tttaaagaag gaaaagttgt ttcttaaacc
     1621 ccgcggggta ccatgtctaa aggtgaagaa ttattcactg gtgttgtccc aattttggtt
     1681 gaattagatg gtgatgttaa tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat
     1741 gctacttacg gtaaattgac cttaaaattt atttgtacta ctggtaaatt gccagttcca
     1801 tggccaacct tagtcactac tttaacttat ggtgttcaat gtttttctag atacccagat
     1861 catatgaaac aacatgactt tttcaagtct gccatgccag aaggttatgt tcaagaaaga
     1921 actatttttt tcaaagatga cggtaactac aagaccagag ctgaagtcaa gtttgaaggt
     1981 gataccttag ttaatagaat cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt
     2041 ttaggtcaca aattggaata caactataac tctcacaatg tttacatcat ggctgacaaa
     2101 caaaagaatg gtatcaaagt taacttcaaa attagacaca acattgaaga tggttctgtt
     2161 caattagctg accattatca acaaaatact ccaattggtg atggtccagt cttgttacca
     2221 gacaaccatt acttatccac tcaatctgcc ttatccaaag atccaaacga aaagagagac
     2281 cacatggtct tgttagaatt tgttactgct gctggtatta cccatggtat ggatgaattg
     2341 tacaaagaga tctcaatcag tctgattgcg gcgttagcgg tagatcacgt tatcggcatg
     2401 gaaaacgcca tgccgtggaa cctgcctgcc gatctcgcct ggtttaaacg caacacctta
     2461 aataaacccg tgattatggg ccgccatacc tgggaatcaa tcggtcgtcc gttgccagga
     2521 cgcaaaaata ttatcctcag cagtcaaccg agtacggacg atcgcgtaac gtgggtgaag
     2581 tcggtggatg aagccatcgc ggcgtgtggt gacgtaccag aaatcatggt gattggcggc
     2641 ggtcgcgttt atgaacagtt cttgccaaaa gcgcaaaaac tgtatctgac gcatatcgac
     2701 gcagaagtgg aaggcgacac ccatttcccg gattacgagc cggatgactg ggaatcggta
     2761 ttcagcgaat tccacgatgc tgatgcgcag aactctcaca gctattgctt tgagattctg
     2821 gagcggcgat aactcgagct gcagatgaat cgtagatact gaaaaacccc gcaagttcac
     2881 ttcaactgtg catcgtgcac catctcaatt tctttcattt atacatcgtt ttgccttctt
     2941 ttatgtaact atactcctct aagtttcaat cttggccatg taacctctga tctatagaat
     3001 tttttaaatg actagaatta atgcccatct tttttttgga cctaaattct tcatgaaaat
     3061 atattacgag ggcttattca gaagctttgg acttcttcgc cagaggtttg gtcaagtctc
     3121 caatcaaggt tgtcggcttg tctaccttgc cagaaattta cgaaaagatg gaaaagggtc
     3181 aaatcgttgg tagatacgtt gttgacactt ctaaataagc gaatttctta tgatttatga
     3241 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt
     3301 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt
     3361 ctcaggtata gcatgaggtc gctcttattg accacacctc taccggccgg tcgaaattcc
     3421 cctaccctat gaacatattc cattttgtaa tttcgtgtcg tttctattat gaatttcatt
     3481 tataaagttt atgtacaaat atcataaaaa aagagaatct ttttaagcaa ggattttctt
     3541 aacttcttcg gcgacagcat caccgacttc ggtggtactg ttggaaccac ctaaatcacc
     3601 agttctgata cctgcatcca aaaccttttt aactgcatct tcaatggcct taccttcttc
     3661 aggcaagttc aatgacaatt tcaacatcat tgcagcagac aagatagtgg cgatagggtt
     3721 gaccttattc tttggcaaat ctggagcaga accgtggcat ggttcgtaca aaccaaatgc
     3781 ggtgttcttg tctggcaaag aggccaagga cgcagatggc aacaaaccca aggaacctgg
     3841 gataacggag gcttcatcgg agatgatatc accaaacatg ttgctggtga ttataatacc
     3901 atttaggtgg gttgggttct taactaggat catggcggca gaatcaatca attgatgttg
     3961 aaccttcaat gtaggaaatt cgttcttgat ggtttcctcc acagtttttc tccataatct
     4021 tgaagaggcc aaaacattag ctttatccaa ggaccaaata ggcaatggtg gctcatgttg
     4081 tagggccatg aaagcggcca ttcttgtgat tctttgcact tctggaacgg tgtattgttc
     4141 actatcccaa gcgacaccat caccatcgtc ttcctttctc ttaccaaagt aaatacctcc
     4201 cactaattct ctgacaacaa cgaagtcagt acctttagca aattgtggct tgattggaga
     4261 taagtctaaa agagagtcgg atgcaaagtt acatggtctt aagttggcgt acaattgaag
     4321 ttctttacgg atttttagta aaccttgttc aggtctaaca ctacctgtac cccatttagg
     4381 accacccaca gcacctaaca aaacggcatc aaccttcttg gaggcttcca gcgcctcatc
     4441 tggaagtggg acacctgtag cgtcgatagc agcaccacca attaaatgat tttcgaaatc
     4501 gaacttgaca ttggaacgaa catcagaaat agctttaaga accttaatgg cttcggctgt
     4561 gatttcttga ccaacgtggt cacctggcaa aacgacgatc ttcttagggg cagacattag
     4621 aatggtatat ccttgaaata tatatatata ttgctgaaat gtaaaaggta agaaaagtta
     4681 gaaagtaaga cgattgctaa ccacctattg gaaaaaacaa taggtcctta aataatattg
     4741 tcaacttcaa gtattgtgat gcaagcattt agtcatgaac gcttctctat tctatatgaa
     4801 aagccggttc cggcgctctc acctttcctt tttctcccaa tttttcagtt gaaaaaggta
     4861 tatgcgtcag gcgacctctg aaattaacaa aaaatttcca gtcatcgaat ttgattctgt
     4921 gcgatagcgc ccctgtgtgt tctcgttatg ttgaggaaaa aaataatggt tgctaagaga
     4981 ttcgaactct tgcatcttac gatacctgag tattcccaca gttggggatc tcgactctag
     5041 ctagaggatc aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5101 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5161 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5221 gtcgtgccag ctgataactt cgtataatgt atgctatacg aagttattag gtctgaagag
     5281 gagtttacgt ccagccaagc tagcttggct gcaggtcgag cggccgcgat ccggaaccct
     5341 taatataact tcgtataatg tatgctatac gaagttatca gctgcattaa tgaatcggcc
     5401 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5461 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5521 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5581 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5641 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5701 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5761 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5821 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5881 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5941 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6001 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6061 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6121 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6181 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6241 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6301 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6361 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6421 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6481 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6541 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6601 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6661 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6721 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6781 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6841 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6901 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6961 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7021 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7081 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7141 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7201 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7261 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7321 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     7381 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     7441 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     7501 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     7561 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     7621 ataacgcatt taagcataaa cacgcactat gccgttcttc tcatgtatat atatatacag
     7681 gcaacacgca gatataggtg cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc
     7741 attttcggaa gcgctcgttt tcggaaacgc tttgaagttc ctattccgaa gttcctattc
     7801 tctagctaga aagtatagga acttcagagc gcttttgaaa accaaaagcg ctctgaagac
     7861 gcactttcaa aaaaccaaaa acgcaccgga ctgtaacgag ctactaaaat attgcgaata
     7921 ccgcttccac aaacattgct caaaagtatc tctttgctat atatctctgt gctatatccc
     7981 tatataacct acccatccac ctttcgctcc ttgaacttgc atctaaactc gacctctaca
     8041 ttttttatgt ttatctctag tattactctt tagacaaaaa aattgtagta agaactattc
     8101 atagagtgaa tcgaaaacaa tacgaaaatg taaacatttc ctatacgtag tatatagaga
     8161 caaaatagaa gaaaccgttc ataattttct gaccaatgaa gaatcatcaa cgctatcact
     8221 ttctgttcac aaagtatgcg caatccacat cggtatagaa tataatcggg gatgccttta
     8281 tcttgaaaaa atgcacccgc agcttcgcta gtaatcagta aacgcgggaa gtggagtcag
     8341 gcttttttta tggaagagaa aatagacacc aaagtagcct tcttctaacc ttaacggacc
     8401 tacagtgcaa aaagttatca agagactgca ttatagagcg cacaaaggag aaaaaaagta
     8461 atctaagatg ctttgttaga aaaatagcgc tctcgggatg catttttgta gaacaaaaaa
     8521 gaagtataga ttctttgttg gtaaaatagc gctctcgcgt tgcatttctg ttctgtaaaa
     8581 atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt ttgttttaca
     8641 aaaatgaagc acagattctt cgttggtaaa atagcgcttt cgcgttgcat ttctgttctg
     8701 taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg catttttgtt
     8761 ctacaaaatg aagcacagat gcttcgttgc t
//
