LOCUS       sequence-114383-       14084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible expression of IDH2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14084)
  AUTHORS   Lewis CA, Parker SJ, Fiske BP, McCloskey D, Gui DY, Green CR, Vokes 
            NI, Feist AM, Vander Heiden MG, Metallo CM
  TITLE     Tracing compartmentalized NADPH metabolism in the cytosol and 
            mitochondria of mammalian cells.
  JOURNAL   Mol Cell. 2014 Jul 17;55(2):253-63. doi: 
            10.1016/j.molcel.2014.05.008. Epub 2014 May 29.
  PUBMED    24882210
REFERENCE   2  (bases 1 to 14084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.05.008"; journalName: "Mol Cell"; date: 
            "2014-07-17- 17"; volume: "55"; issue: "2"; pages: "253-63"
COMMENT     Sequence Label: pSLIK-IDH2-FLAG
FEATURES             Location/Qualifiers
     source          1..14084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2991..3000
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4374..4439
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(4460..4484)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4534..5745
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5582..5601
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5870..6487,6488..6493,6494..6529,6530..6532,6533..6568,
                     6569..6571,6572..6607,6608..6616)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6654..7228
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6821..6838)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             7287..8306
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             7287..8306
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    8772..9360
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8825..8845)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9243..9254)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9363..9379)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9364..9380)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9889..10069
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10095..10112)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10101..10325
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10371..10799
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10458..10477)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10668..10689
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10808..10828)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10813..11142
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10993..11128
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11055..11074
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11190..11237
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11256..11630
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11760..11881
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11797..11816)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11851..11870
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11930..11946)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11930..11946)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11943..11965)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11954..11970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11978..11984,11985..12002,12003..12008))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12023..12044
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12161..12178)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12332..12920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12412..12431)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13091..13882,13883..13951))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13714..13733
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13952..14056)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactagt gccaccatgg
     3001 ccggctacct gcgggtcgtg cgctcgctct gcagagcctc aggctcgcgg ccggcctggg
     3061 cgccggcggc cctgacagcc cccacctcgc aagagcagcc gcggcgccac tatgccgaca
     3121 aaaggatcaa ggtggcgaag cccgtggtgg agatggatgg tgatgagatg acccgtatta
     3181 tctggcagtt catcaaggag aagctcatcc tgccccacgt ggacatccag ctaaagtatt
     3241 ttgacctcgg gctcccaaac cgtgaccaga ctgatgacca ggtcaccatt gactctgcac
     3301 tggccaccca gaagtacagt gtggctgtca agtgtgccac catcacccct gatgaggccc
     3361 gtgtggaaga gttcaagctg aagaagatgt ggaaaagtcc caatggaact atccggaaca
     3421 tcctgggggg gactgtcttc cgggagccca tcatctgcaa aaacatccca cgcctagtcc
     3481 ctggctggac caagcccatc accattggca ggcacgccca tggcgaccag tacaaggcca
     3541 cagactttgt ggcagaccgg gccggcactt tcaaaatggt cttcacccca aaagatggca
     3601 gtggtgtcaa ggagtgggaa gtgtacaact tccccgcagg cggcgtgggc atgggcatgt
     3661 acaacaccga cgagtccatc tcaggttttg cgcacagctg cttccagtat gccatccaga
     3721 agaaatggcc gctgtacatg agcaccaaga acaccatact gaaagcctac gatgggcgtt
     3781 tcaaggacat cttccaggag atctttgaca agcactataa gaccgacttc gacaagaata
     3841 agatctggta tgagcaccgg ctcattgatg acatggtggc tcaggtcctc aagtcttcgg
     3901 gtggctttgt gtgggcctgc aagaactatg acggagatgt gcagtcagac atcctggccc
     3961 agggctttgg ctcccttggc ctgatgacgt ccgtcctggt ctgccctgat gggaagacga
     4021 ttgaggctga ggccgctcat gggaccgtca cccgccacta tcgggagcac cagaagggcc
     4081 ggcccaccag caccaacccc atcgccagca tctttgcctg gacacgtggc ctggagcacc
     4141 gggggaagct ggatgggaac caagacctca tcaggtttgc ccagatgctg gagaaggtgt
     4201 gcgtggagac ggtggagagt ggagccatga ccaaggacct ggcgggctgc attcacggcc
     4261 tcagcaatgt gaagctgaac gagcacttcc tgaacaccac ggacttcctc gacaccatca
     4321 agagcaacct ggacagagcc ctgggcaggc agtattctag aggatcccgg gctgactaca
     4381 aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt
     4441 agtgactcga gatatctaga cccagctttc ttgtacaaag tggttgataa catctcgagc
     4501 cataaagatg gttaattaac ccacccaaga tctggcctcc gcgccgggtt ttggcgcctc
     4561 ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc
     4621 gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct
     4681 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac
     4741 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg
     4801 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
     4861 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg
     4921 atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc
     4981 gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc
     5041 aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc
     5101 ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg
     5161 gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg
     5221 gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga
     5281 actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc
     5341 cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat
     5401 aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg
     5461 cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg
     5521 gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag
     5581 ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt
     5641 taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt
     5701 aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttggtaccga
     5761 gctcggatct ccaccccgta ccggtcctgc aggatttaaa tggcgcgccg ctagcgctac
     5821 cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gaattcacca tgtctagact
     5881 ggacaagagc aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg
     5941 cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca
     6001 cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca
     6061 tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa
     6121 gtcataccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg
     6181 cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg
     6241 cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg
     6301 cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga
     6361 ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc
     6421 tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga
     6481 aagcggcggg ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc
     6541 ccttgacgac tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga
     6601 catgctcccc gggtaactaa gtaaggatcc gcggccgcac tagaggaatt ccgcccctct
     6661 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgtgttt
     6721 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     6781 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     6841 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     6901 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     6961 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     7021 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtagtcaa caaggggctg
     7081 aaggatgccc agaaggtacc ccattgtatg ggaatctgat ctggggcctc ggtgcacatg
     7141 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     7201 ggttttcctt tgaaaaacac gatgataagc ttaccggtac gcgtgatagc ttgccacaac
     7261 ccgtaccaaa gatggataga tccggaaagc ctgaactcac cgcgacgtct gtcgagaagt
     7321 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     7381 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     7441 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     7501 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     7561 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     7621 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     7681 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     7741 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     7801 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     7861 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     7921 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     7981 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     8041 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     8101 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     8161 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     8221 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     8281 gcactcgtcc gagggcaaag gaatagagta gatgccgacc gaacaagagc tgatttcgag
     8341 aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa atgcgagaga acggccttac
     8401 gcttggtggc acagttctcg tccacagttc gctaagctcg ctcggctggg tcgcgggagg
     8461 gccggtcgca gtgattcagg cccttctgga ttgtgttggt ccccagggca cgattgtcat
     8521 gcccacgcac tcgggtgatc tgactgatcc cgcagattgg agatcgccgc ccgtgcctgc
     8581 cgattgggtg cagatctcta gatctgtaca agtaaagcgg ccgcgactct agatcataat
     8641 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     8701 gaacctgaaa cataaaatga atgcaattgt tgttgtttag tccctcccaa ttcgatatca
     8761 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     8821 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     8881 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     8941 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     9001 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     9061 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     9121 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     9181 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     9241 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     9301 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     9361 atcgataccg tcgacctcga tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     9421 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     9481 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     9541 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     9601 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     9661 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
     9721 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
     9781 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
     9841 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
     9901 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     9961 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
    10021 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta
    10081 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
    10141 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
    10201 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
    10261 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
    10321 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
    10381 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
    10441 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
    10501 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
    10561 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
    10621 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
    10681 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
    10741 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
    10801 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
    10861 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
    10921 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
    10981 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    11041 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
    11101 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
    11161 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
    11221 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
    11281 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
    11341 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
    11401 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
    11461 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
    11521 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
    11581 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
    11641 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
    11701 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
    11761 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    11821 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    11881 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
    11941 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    12001 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    12061 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    12121 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    12181 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    12241 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    12301 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    12361 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    12421 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    12481 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    12541 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    12601 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    12661 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    12721 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    12781 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    12841 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    12901 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    12961 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    13021 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    13081 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    13141 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    13201 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    13261 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    13321 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    13381 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    13441 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    13501 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    13561 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    13621 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    13681 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    13741 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    13801 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    13861 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    13921 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    13981 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    14041 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
