LOCUS       sequence-114384-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of frameshift mutated Cx43 and EGFP separately 
            driven by the ECMV IRES. For transfection or retroviral production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Salat-Canela C, Sese M, Peula C, Ramon y Cajal S, Aasen T
  TITLE     Internal translation of the connexin 43 transcript.
  JOURNAL   Cell Commun Signal. 2014 May 8;12:31. doi: 10.1186/1478-811X-12-31.
  PUBMED    24884945
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Commun
            Signal."; date: "2014-05-8"; volume: "12:31. doi"; pages: " 
            10.1186/1478-811X-12-31"
COMMENT     Sequence Label: pLPCX-Cx43-FS-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..78
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     63..79
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgaggtcg acggtatcga taagcttgat atcaacaagt ttgtacaaaa aagcaggctt
      121 catgggtgac tggagcgcct taggcaaact ccttgcaagg ttcaagccta ctcaactgct
      181 ggagggaagg tgtggctgtc agtacttttc attttccgaa tcctgctgct ggggacagcg
      241 gttgagtcag cctggggaga tgagcagtct gcctttcgtt gtaacactca gcaacctggt
      301 tgtgaaaatg tctgctatga caagtctttc ccaatctctc atgtgcgctt ctgggtcctg
      361 cagatcatat ttgtgtctgt acccacactc ttgtacctgg ctcatgtgtt ctatgtgatg
      421 cgaaaggaag agaaactgaa caagaaagag gaagaactca aggttgccca aactgatggt
      481 gtcaatgtgg acatgcactt gaagcagatt gagataaaga agttcaagta cggtattgaa
      541 gagcatggta aggtgaaaat gcgagggggg ttgctgcgaa cctacatcat cagtatcctc
      601 ttcaagtcta tctttgaggt ggccttcttg ctgatccagt ggtacatcta tggattcagc
      661 ttgagtgctg tttacacttg caaaagagat ccctgcccac atcaggtgga ctgtttcctc
      721 tctcgcccca cggagaaaac catcttcatc atcttcatgc tggtggtgtc cttggtgtcc
      781 ctggccttga atatcattga actcttctat gttttcttca agggcgttaa ggatcgggtt
      841 aagggaaaga gcgaccctta ccatgcgacc agtggtgcgc tgagccctgc caaagactgt
      901 gggtctcaaa aatat
//
