LOCUS       sequence-114393-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Plasma Membrane, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3..12
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9..740
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 tcgccaccat ggtgagcaag ggcgaggagc tgatcaagga gaacatgcac atgaagctgt
       61 acatggaagg caccgtgaac aaccaccact tcaagtgcac caccgaaggg gagggcaagc
      121 cctacgaggg cacccagacc cagaggatta aggtggtgga gggaggcccc ctgccgttcg
      181 cattcgacat cctggccacc tgctttatgt acgggagcaa gaccttcatc aaccacaccc
      241 agggcatccc cgatttcttt aagcagtcct tccctgaggg cttcacatgg gagagagtca
      301 ccacatacga agacgggggc gtgcttaccg ttacccagga caccagcctc caggacggct
      361 gcttgatcta caacgtcaag ctcagagggg tgaacttccc atccaacggc cctgtgatgc
      421 agaagaaaac actcggctgg gaggccacca ccgagaccct gtaccccgct gacggcggcc
      481 tggaaggcag atgcgacatg gccctgaagc tcgtgggcgg gggccacctg cactgcaacc
      541 tgaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc ggcgtctact
      601 ttgtggaccg cagactggaa agaatcaagg aggccgacaa tgagacctac gtcgagcagc
      661 acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac aaacttaatg
      721 gcatggacga gctgtacaag tccggactca gatctaagct gaaccctcct gatgagagtg
      781 gccccggctg catgagctgc aagtgtgtgc tctcctgaga attctgcagt cgacggt
//
