LOCUS       sequence-114395-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of frameshift mutated Cx43 and EGFP separately 
            driven by the ECMV IRES. For transfection or retroviral production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Salat-Canela C, Sese M, Peula C, Ramon y Cajal S, Aasen T
  TITLE     Internal translation of the connexin 43 transcript.
  JOURNAL   Cell Commun Signal. 2014 May 8;12:31. doi: 10.1186/1478-811X-12-31.
  PUBMED    24884945
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Commun
            Signal."; date: "2014-05-8"; volume: "12:31. doi"; pages: " 
            10.1186/1478-811X-12-31"
COMMENT     Sequence Label: pLPCX-Cx43-FS-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(153..155,156..158,159..872)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(153..155,156..158,159..869)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(198..219)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(459..478)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     806..827
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccagaaggta ccccattgta tgggatctga tctggggcct cggtacacat gctttacatg
       61 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
      121 ttgaaaaaca cgatgataat atggccacaa ccatggtgag caagggcgag gagctgttca
      181 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      241 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      301 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      361 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      421 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      481 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      541 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      601 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
      661 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      721 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
      781 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      841 tcactctcgg catggacgag ctgtacaagt aaagcggccg cctcggccaa acatcgataa
      901 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
      961 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaa
//
