LOCUS       sequence-114396-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation:N/A, 
            Emission:N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-TagRFP-pBad
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             93..110
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             114..146
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             159..179
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gcaactctct actggtttct ccatacccgt ttttttgggc tagaaataat tttgtttaac
       61 tttaagaagg agatatacat atgcggggtt ctcatcatca tcatcatcat ggtatggcta
      121 gcatgactgg tggacagcaa atgggtcggg atctgtacga gaacctgtac ttccagggct
      181 cgagcatggt gtctaagggc gaagagctga ttaaggagaa catgcacatg aagctgtaca
      241 tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct
      301 acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct
      361 tcgacatcct ggctaccagc ttcatgtacg gcagcagcac cttcatcaac cacacccagg
      421 gcatccccga cttctggaag cagtccttcc ctgagggctt cacatgggag agagtcacca
      481 catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
      541 tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgaaga
      601 agaaaacact cggctgggaa ccgagcaccg agaaactgaa acccgctgac ggcggcctgg
      661 aaggcagggt cgacatggcc
//
