LOCUS       sequence-114400-         560 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for the human AAVS1 locus for insertion of traffic 
            light reporter (TLR) construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198."
COMMENT     Sequence Label: pAAVS1-TLR targeting vector
FEATURES             Location/Qualifiers
     source          1..560
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(61..78)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    67..291
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggtgcatgac ccgcaagccc ggtgcctgat ctagagggcc cgtttaaacc cgctgatcag
       61 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
      121 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
      181 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
      241 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg ggtcgacggt
      301 atcgataagc ttgatatcga attcgagctc ggtacccggg ggcgcgccgg attcgacatt
      361 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      421 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      481 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      541 attgacgtca atgggtggag
//
