LOCUS       sequence-114405-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning, T7 RNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Salat-Canela C, Sese M, Peula C, Ramon y Cajal S, Aasen T
  TITLE     Internal translation of the connexin 43 transcript.
  JOURNAL   Cell Commun Signal. 2014 May 8;12:31. doi: 10.1186/1478-811X-12-31.
  PUBMED    24884945
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Commun
            Signal."; date: "2014-05-8"; volume: "12:31. doi"; pages: " 
            10.1186/1478-811X-12-31"
COMMENT     Sequence Label: pBS-Cx43
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     194..216
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     208..225
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     209..225
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     235..254
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        235..253
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     278..294
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     279..295
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    311..335
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
       61 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
      121 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
      181 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
      241 gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcgacgg tatcgataag
      301 cttgatatca acaagtttgt acaaaaaagc aggcttcatg ggtgactgga gcgccttagg
      361 caaactcctt gacaaggttc aagcctactc aactgctgga gggaaggtgt ggctgtcagt
      421 acttttcatt ttccgaatcc tgctgctggg gacagcggtt gagtcagcct ggggagatga
      481 gcagtctgcc tttcgttgta acactcagca acctggttgt gaaaatgtct gctatgacaa
      541 gtctttccca atctctcatg tgcgcttctg ggtcctgcag atcatatttg tgtctgtacc
      601 cacactcttg tacctggctc atgtgttcta tgtgatgcga aaggaagaga aactgaacaa
      661 gaaagaggaa gaactcaagg ttgcccaaac tgatggtgtc aatgtggaca tgcacttgaa
      721 gcagattgag ataaagaagt tcaagtacgg tattgaagag catggtaagg tgaaaatgcg
      781 aggggggttg ctgcgaacct acatcatcag tatcctcttc aagtctatct ttgaggtggc
      841 cttcttgctg atccagtggt acatctatgg attcagcttg agtgctgttt acacttgcaa
      901 aagagatccc tgcccacatc aggtggactg tttcctctct cgccccacgg agaaaaccat
      961 cttcatcatc ttcatgctgg tggtgtcctt ggtgtccctg gccttga
//
