LOCUS       sequence-114407-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP 
            expression cassette - Ampicillin plasmid 
            [E:J23102:B:BCD2:C:E0040m:D:B0015:F].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: pJ02B2Gm_EF
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..89
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        108..142
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     RBS             217..225
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(265..293)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaga ctagtgggtc tcagcttttg acagctagct
      121 cagtcctagg tactgtgcta gctactgggc ccaagttcac ttaaaaagga gatcaacaat
      181 gaaagcaatt ttcgtactga aacatcttaa tcatgctaag gaggttttct aatgatgcgt
      241 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
      301 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
      361 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
      421 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
      481 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
      541 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
      601 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
      661 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
      721 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
      781 caacaa
//
