LOCUS       sequence-114411-        3049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP 
            expression cassette - Ampicillin plasmid 
            [E:J23102:B:BCD2:C:E1010m:D:B0015:F].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: pJ02B2Rm_EF
FEATURES             Location/Qualifiers
     source          1..3049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..39
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     RBS             114..122
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             132..809
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      825..896
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      912..939
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    957..977
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(983..1000)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1154..1742)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1234..1253)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1913..2704,2705..2773))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2536..2555
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2774..2878)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2946..2964
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2968..3011)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3014..3035
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gcttttgaca gctagctcag tcctaggtac tgtgctagct actgggccca agttcactta
       61 aaaaggagat caacaatgaa agcaattttc gtactgaaac atcttaatca tgctaaggag
      121 gttttctaat gatggcttcc tccgaggatg ttatcaaaga gttcatgcgt ttcaaagttc
      181 gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc
      241 cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg
      301 cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg
      361 ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta
      421 tgaacttcga agatggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg
      481 agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc
      541 agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggag gatggtgctc
      601 tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag
      661 ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca
      721 tcaaactgga catcacctcc cacaacgagg actacaccat cgttgaacag tacgaacgtg
      781 ctgaaggtcg tcactccacc ggtgcttaat aaaggtccag gcatcaaata aaacgaaagg
      841 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      901 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatacgcta gagacctact
      961 agtagcggcc gctgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1021 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1081 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1141 cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1201 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1261 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1321 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1381 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1441 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1501 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1561 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1621 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1681 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1741 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1801 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1861 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1921 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1981 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2041 caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2101 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2161 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2221 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2281 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2341 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2401 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2461 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2521 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2581 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2641 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2701 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2761 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2821 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2881 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     2941 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta
     3001 aggatgattt ctggaattcg cggccgcttc tagagactag tgggtctca
//
