LOCUS       sequence-114413-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning, T7 RNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Salat-Canela C, Sese M, Peula C, Ramon y Cajal S, Aasen T
  TITLE     Internal translation of the connexin 43 transcript.
  JOURNAL   Cell Commun Signal. 2014 May 8;12:31. doi: 10.1186/1478-811X-12-31.
  PUBMED    24884945
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Commun
            Signal."; date: "2014-05-8"; volume: "12:31. doi"; pages: " 
            10.1186/1478-811X-12-31"
COMMENT     Sequence Label: pBS-Cx43-FS
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    128..149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(164..169,170..187,188..194)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    202..218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     207..229
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     226..242
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     226..242
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     261..281
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        263..281
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     318..334
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     318..334
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    361..385
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
       61 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
      121 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      181 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
      241 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctcc
      301 accgcggtgg cggccgctct agaactagtg gatcccccgg gctgcaggaa ttcgatatca
      361 accactttgt acaagaaagc tgggtactag atctccaggt catcaggccg aggtctgctg
      421 ctggcacgac tgctggctct gcttgaaggt cgctggtcca caatggctag tggctgtaat
      481 tcatgtccag cagctagttt ttttgaattc tggttatcat cggggaaatc aaaaggctgt
      541 gcatgggagt tagagatggt gcttcccgcc tgccccattc gattttgttc tgcactgtaa
      601 ttagcccagt tttgctcact tgcttgcttg ttgtaattgc ggcaagaaga attgtttctg
      661 tcgccagtaa ccagcttgta cccaggagga gacataggcg agaggggagc ggttggtgag
      721 gagcagccat tgaaataagc atatttttga gacccacagt ctttggcagg gctcagcgca
      781 ccactggtcg catggtaagg gtcgctcttt cccttaaccc gatccttaac gcccttgaag
      841 aaaacataga agagttcaat gatattcaag gccagggaca ccaaggacac caccagcatg
      901 aagatgatga agatggtttt ctccgtgggg cgagagagga aacagtccac ctgatgtggg
      961 cagggatctc ttttgcaagt gtaaacagca ctca
//
