LOCUS       sequence-114415-       15320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lox-STOP-Lox reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15320)
  AUTHORS   Li Y, Park A, Mou H, Colpan C, Bizhanova A, Akama-Garren E, Joshi N,
            Hendrickson EA, Feldser D, Yin H, Anderson DG, Jacks T, Weng Z, Xue 
            W
  TITLE     A versatile reporter system for CRISPR-mediated chromosomal 
            rearrangements.
  JOURNAL   Genome Biol. 2015 May 28;16(1):111.
  PUBMED    26018130
REFERENCE   2  (bases 1 to 15320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-05-28"; volume: "16(1)"; pages: "111"
COMMENT     Sequence Label: LSL
FEATURES             Location/Qualifiers
     source          1..15320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        74..178
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(179..247,248..1039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(397..416)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1210..1798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1699..1718
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1952..1969
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2086..2107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2122..2127,2128..2145,2146..2152)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2160..2176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2165..2187
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2184..2200
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2184..2200
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2219..2239
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2221..2239
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    3107..3231
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3256..3261,3262..3279,3280..3286)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3340..3999
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3406..3425)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4341..4646
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4525..4544
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4687..4811)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     enhancer        4854..5233
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5235..5512
                     /label=chicken beta-actin promoter
     intron          5514..6531
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6455..6477
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6539..6558
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     6591..6607
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6621..6654
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6684..7180
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6719..6742)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             7194..7997
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7257..7276)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7867..7886
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8007..8468
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     polyA_signal    8616..8750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    8870..9004
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    9124..9258
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    9287..9320
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(9356..9973,9974..9979,9980..10015,10016..10018,
                     10019..10054,10055..10057,10058..10093,10094..10099)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             10115..10168
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             10175..11602
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             11618..11677
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             11684..13333
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             13340..13387
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             13391..13510
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    13555..13676
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13774..13790)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(13800..13847)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    13898..14708
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(14726..14745)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14727..14745)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14755..14772)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14755..14771)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14764..14786)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(14913..15320,1..48)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15000..15019)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15210..15231
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
       61 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      121 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      181 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      241 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      301 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      361 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      421 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      481 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      541 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      601 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      661 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      721 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      781 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      841 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
      901 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
      961 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1021 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1081 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1141 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1201 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1261 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1321 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1381 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1441 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1501 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1561 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1621 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1681 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1741 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1801 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1861 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     1921 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     1981 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     2041 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     2101 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     2161 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     2221 aattaaccct cactaaaggg aacaaaagct ggagctctgt tttggttggc gtaaggcgcc
     2281 tgtcagttaa cggcagccgg agtgcgcagc cgccggcagc ctcgctctgc ccactgggtg
     2341 gggcgggagg taggtggggt gaggcgagct ggacgtgcgg gcgcggtcgg cctctggcgg
     2401 ggcgggggag gggagggagg gtcagcgaaa gtagctcgcg cgcgagcggc cgcccaccct
     2461 ccccttcctc tgggggagtc gttttacccg ccgccggccg ggcctcgtcg tctgattggc
     2521 tctcggggcc cagaaaactg gcccttgcca ttggctcgtg ttcgtgcaag ttgagtccat
     2581 ccgccggcca gcgggggcgg cgaggaggcg ctcccaggtt ccggccctcc cctcggcccc
     2641 gcgccgcaga gtctggccgc gcgcccctgc gcaacgtggc aggaagcgcg cgctgggggc
     2701 ggggacgggc agtagggctg agcggctgcg gggcgggtgc aagcacgttt ccgacttgag
     2761 ttgcctcaag aggggcgtgc tgagccagac ctccatcgcg cactccgggg agtggaggga
     2821 aggagcgagg gctcagttgg gctgttttgg aggcaggaag cacttgctct cccaaagtcg
     2881 ctctgagttg ttatcagtaa gggagctgca gtggagtagg cggggagaag gccgcaccct
     2941 tctccggagg ggggagggga gtgttgcaat acctttctgg gagttctctg ctgcctcctg
     3001 gcttctgagg accgccctgg gcctgggaga atcccttccc cctcttccct cgtgatctgc
     3061 aactccagtc tttctcccgg gagagtcgag gaattctgca gatatcacaa gtttgtacaa
     3121 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
     3181 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca
     3241 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt
     3301 taggatccgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     3361 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     3421 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     3481 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     3541 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
     3601 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     3661 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     3721 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
     3781 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     3841 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     3901 gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa
     3961 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta
     4021 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
     4081 gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat gaagcagcgt attacagtga
     4141 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
     4201 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
     4261 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
     4321 cgagaacagg gactggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
     4381 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
     4441 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     4501 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     4561 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     4621 ttaacctgat gttctgggga atataaatgt caggctccgt tatacacagc cagtctgcag
     4681 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
     4741 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg
     4801 tacaaagtgg tgataaaccc gctgatcagc tcgacgttta aacctcgaga ttcgacattg
     4861 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     4921 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     4981 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     5041 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     5101 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     5161 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     5221 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
     5281 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
     5341 gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
     5401 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
     5461 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cggggagtcg
     5521 ctgcgacgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
     5581 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     5641 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
     5701 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
     5761 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
     5821 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
     5881 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     5941 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     6001 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     6061 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     6121 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
     6181 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     6241 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     6301 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     6361 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     6421 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     6481 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     6541 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattggccgc tctagaacta
     6601 gtggatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc
     6661 cctcgacctg caggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     6721 atgcgcttta gcagccccgc tggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     6781 cattccacat ccaccggtag cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     6841 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     6901 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     6961 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     7021 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     7081 gggcgggcgc gaaggtcctc ccgaggcccg gcattctcgc acgcttcaaa agcgcacgtc
     7141 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc aatatgggat
     7201 cggccattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     7261 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     7321 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     7381 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     7441 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     7501 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     7561 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     7621 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     7681 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     7741 acggcgatga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     7801 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     7861 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     7921 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     7981 ttgacgagtt cttctgaggg gatccgctgt aagtctgcag aaattgatga tctattaaac
     8041 aataaagatg tccactaaaa tggaagtttt tcctgtcata ctttgttaag aagggtgaga
     8101 acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga
     8161 ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca
     8221 tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca
     8281 ctcatgatct atagatctat agatctctcg tgggatcatt gtttttctct tgattcccac
     8341 tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga
     8401 tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa gttctaattc
     8461 catcagaagc ttgcagatct gcgactctag aggatctgcg actctagagg atcataatca
     8521 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     8581 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     8641 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8701 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgcgactcta
     8761 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     8821 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     8881 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8941 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9001 gatctgcgac tctagaggat cataatcagc cataccacat ttgtagaggt tttacttgct
     9061 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     9121 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9181 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9241 tcttatcatg tctggatccc catcaagctg atccggaacc cttaatataa cttcgtataa
     9301 tgtatgctat acgaagttat taggtccctc gacctgcagc ccaagctagg ccaccatgtc
     9361 tagactggac aagagcaaag tcataaacgg agctctggaa ttactcaatg gtgtcggtat
     9421 cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta
     9481 ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag
     9541 gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa
     9601 cgccaagtca taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg
     9661 cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca
     9721 gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact
     9781 gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac
     9841 caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc
     9901 cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa
     9961 gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc
    10021 cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga
    10081 ccttgacatg ctccccgggg gaagtggagc tagggagggc agaggaagtc ttctaacatg
    10141 cggtgacgtg gaggagaatc ccggccctgc tagaatggtg agcaagggcg aggaggtcat
    10201 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
    10261 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
    10321 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
    10381 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
    10441 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
    10501 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
    10561 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
    10621 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
    10681 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
    10741 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
    10801 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg
    10861 caccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc
    10921 cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga
    10981 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
    11041 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat
    11101 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc
    11161 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac
    11221 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg
    11281 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
    11341 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
    11401 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc
    11461 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
    11521 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta
    11581 cggcatggac gagctgtaca agggaagtgg atctagccag tgtactaatt atgctctctt
    11641 gaaattggct ggagatgttg agagcaaccc aggtccctct aggatggaag atgccaaaaa
    11701 cattaagaag ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct
    11761 gcacaaagcc atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca
    11821 tatcgaggtg gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc
    11881 tatgaagcgc tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt
    11941 gcagttcttc atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa
    12001 cgacatctac aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt
    12061 attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat
    12121 acaaaagatc atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac
    12181 cttcgtgact tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt
    12241 cgaccgggac aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa
    12301 gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat
    12361 cttcggcaac cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg
    12421 cttcggcatg ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta
    12481 ccgcttcgag gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct
    12541 gctggtgccc acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct
    12601 aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc
    12661 cgtggccaaa cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac
    12721 cagcgccatt ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt
    12781 gcccttcttc gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca
    12841 gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga
    12901 ggctacaaac gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg
    12961 ggacgaggac gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg
    13021 ctaccaggta gccccagccg aactggagag catcctgctg caacacccca acatcttcga
    13081 cgccggggtc gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt
    13141 gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt
    13201 tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact
    13261 gaccggcaag ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg
    13321 caagatcgcc gtgaattctg cttgcaagaa ctggttcagt agcttaagcc actttgtgat
    13381 ccaccttaac agccacggct tccctcccga ggtggaggag caggccgccg gcaccctgcc
    13441 catgagctgc gcccaggaga gcggcatgga tagacaccct gctgcttgcg ccagcgccag
    13501 gatcaacgtc taacctagaa ctagcttatc gatggccgct tcgagcagac atgataagat
    13561 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
    13621 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
    13681 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
    13741 gcaagtaaaa cctctacaaa tgtggtaaaa tcggatccac tagttctaga gcggccatcg
    13801 aagttcctat actttctaga gaataggaac ttcggaatag taacttctcc ataagcttca
    13861 gctgctcgag accggtggat ccgaattcag accgcggaga tgggcgggag tcttctgggc
    13921 aggcttaaag gctaacctgg tgtgtgggcg ttgtcctgca ggggaattga acaggtgtaa
    13981 aattggaggg acaagacttc ccacagattt tcggttttgt cgggaagttt tttaataggg
    14041 gcaaataagg aaaatgggag gataggtagt catctggggt tttatgcagc aaaactacag
    14101 gttattattg cttgtgatcc gcctcggagt attttccatc gaggtagatt aaagacatgc
    14161 tcacccgagt tttatactct cctgcttgag atccttacta cagtatgaaa ttacagtgtc
    14221 gcgagttaga ctatgtaagc agaattttaa tcatttttaa agagcccagt acttcatatc
    14281 catttctccc gctccttctg cagccttatc aaaaggtatt ttagaacact cattttagcc
    14341 ccattttcat ttattatact ggcttatcca acccctagac agagcattgg cattttccct
    14401 ttcctgatct tagaagtctg atgactcatg aaaccagaca gattagttac atacaccaca
    14461 aatcgaggct gtagctgggg cctcaacact gcagttcttt tataactcct tagtacactt
    14521 tttgttgatc ctttgccttg atccttaatt ttcagtgtct atcacctctc ccgtcaggtg
    14581 gtgttccaca tttgggccta ttctcagtcc agggagtttt acaacaatag atgtattgag
    14641 aatccaacct aaagcttaac tttccactcc catgaatgcc tctctccttt ttctccattt
    14701 ataaactgag ctggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
    14761 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
    14821 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
    14881 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
    14941 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
    15001 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
    15061 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    15121 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    15181 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    15241 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    15301 gttaaaaaat gagctgattt
//
