LOCUS       sequence-114419-       10904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd generation lentiviral transfer plasmid. Expresses mouse CTIP2 
            with blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Richner M, Victor MB, Liu Y, Abernathy D, Yoo AS
  TITLE     MicroRNA-based conversion of human fibroblasts into striatal medium 
            spiny neurons.
  JOURNAL   Nat Protoc. 2015 Oct;10(10):1543-55. doi: 10.1038/nprot.2015.102. 
            Epub 2015 Sep 17.
  PUBMED    26379228
REFERENCE   2  (bases 1 to 10904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.102"; journalName: "Nat Protoc"; date: 
            "2015-10"; volume: "10"; issue: "10"; pages: "1543-55"
COMMENT     Sequence Label: pmCTIP2-N106
FEATURES             Location/Qualifiers
     source          1..10904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5878..6377
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5913..5936)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6310..6329
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6404..6802
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6826..7414
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6879..6899)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7297..7308)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7622..8255
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8384..8400)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8384..8400)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8397..8419)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8408..8424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8432..8438,8439..8456,8457..8462))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8477..8498
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8615..8632)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8786..9371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8866..8885)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9542..10333,10334..10402))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10165..10184
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10403..10507)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10555..10689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10592..10611)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10646..10665
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcgg ccgccaccat gtcccgccgc aaacagggca
     3421 acccgcagca cttgtcccag agggaactca tcacgccaga ggctgaccat gtggaggcta
     3481 ccatcctcga ggaagacgag ggtctggaga tagaggagcc tagcagcctg gggctgatgg
     3541 tgggaggccc cgaccctgat ctactcacct gtggccagtg tcagatgaac ttcccgctgg
     3601 gggacatcct ggtttttata gagcacaaga agaaacagtg tggaggcctg ggcccctgct
     3661 acgacaaggt cctggacaag agcagtccac ctccctcctc tcgctctgag ctcaggagag
     3721 tatctgagcc agtggagatc gggatccagg tcacccctga tgaagatgac cacctactgt
     3781 cacccacgaa aggcatctgt cccaagcagg agaacattgc aggtaaagat gagccttcca
     3841 gctacatttg cacaacatgc aagcagccct tcaacagcgc ctggttcctg ctgcagcacg
     3901 cacagaacac gcatggcttc cgaatctacc tggagcctgg gccggccagc acctcgctca
     3961 cgcccaggct caccatcccg ccaccgctcg ggccggagac cgtggcgcag tccccactca
     4021 tgaatttcct gggggacagc aatcctttca acctgctgcg catgacgggc cccatcctgc
     4081 gggaccaccc tggcttcggt gagggccgct tgccaggtac gccaccgctc ttcagcccac
     4141 cgccacgcca tcacttggac ccacaccgcc tcagtgcaga ggagatgggg ctcgtggccc
     4201 agcaccccag tgccttcgac cgagtcatgc gcctgaaccc catggccata gactctcctg
     4261 ccatggactt ctcccggcgg ctgcgagaac tggccggcaa cagctccacg ccgccgcccg
     4321 tgtccccagg ccgtggcaac cctatgcacc ggctgctgaa ccctttccag cccagtccca
     4381 agtccccgtt cctcagcacg ccaccgctgc cacccatgcc tgcgggcaca ccgccaccgc
     4441 agccgcctgc caagagcaag tcctgtgagt tctgcggcaa gaccttcaag ttccagagca
     4501 atctcatcgt gcaccggcgc agccacacgg gcgagaagcc ctacaagtgc cagctgtgcg
     4561 accatgcgtg ctcgcaggcg agcaagctca agcgccacat gaagacgcac atgcacaagg
     4621 cgggctctct ggctggccgc tcagacgacg ggctctcagc tgccagctcc cctgagccgg
     4681 gcaccagcga gctgccaggt gacctgaaag cggccgatgg cgacttccgc caccatgaga
     4741 gcgacccatc tctgggcccc gagcctgagg acgacgagga cgaggaggag gaagaagagg
     4801 agctgctgct ggagaacgag agccggcctg agtcgagctt cagcatggac tcggagctgg
     4861 gccgtggccg cgagaacgga ggtggtgtgc caccgggggt ggcgggcgca ggggctgcag
     4921 ctgcggctct ggcggatgag aaggctctgg ccctgggcaa ggtgatggag gacgcagggc
     4981 tgggcgcact gccgcagtat ggggagaagc ggggcgcctt cctgaagcgt gcaggcgaca
     5041 cgggtgatgc cggagctgtt ggctgtgggg acgcgggtgc accgggtgca gtgaacgggc
     5101 gcggcggggc cttcgcgcca ggcgcagagc cctttccagc tctcttccca cgcaagccag
     5161 caccgctgcc cagccctggg ctcggtggtc ccgcgctgca cgcggccaag cgcatcaagg
     5221 tggagaaaga cctggagctg ccacctgccg ccctcatccc atctgagaac gtgtactcgc
     5281 agtggctcgt gggctacgca gcatcgcgcc acttcatgaa ggacccattc ctgggcttca
     5341 cggatgcgcg ccagtcgcct ttcgccacat cgtcggaaca ttcctctgag aacggcagcc
     5401 tgcgcttctc aacgccaccc ggggacctgc tggacggcgg gctgtccggg cgcagtggca
     5461 cggcgagcgg gggcagcaca cctcacctgg gtggtccggg tcctgggagg ccgagctcca
     5521 aggagggccg ccgcagcgac acatgtgagt actgcggcaa ggtcttcaag aactgtagca
     5581 acctgacggt gcaccggagg agccacaccg gcgagcggcc ttacaagtgc gagctgtgca
     5641 actacgcgtg tgcgcagagc agcaagctca cgcgccacat gaagacgcac gggcagatcg
     5701 gcaaggaggt gtaccgctgc gacatctgcc agatgccctt cagcgtctac agcaccctgg
     5761 agaaacacat gaaaaagtgg cacggtgaac acttgctgac taatgatgtc aaaatcgagc
     5821 aggctgagag gagctaaggc cgcgccggct ctagatcgcg aacgcgtgaa ttctaccggg
     5881 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     5941 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     6001 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     6061 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     6121 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     6181 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     6241 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     6301 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     6361 catctccggg cctttcgacc tgcagcccaa gcttcctagg accatggcca agcctttgtc
     6421 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     6481 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     6541 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     6601 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     6661 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     6721 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     6781 tggttatgtg tgggagggct aacccgggcg gggcgcgtct ggaacaatca acctctggat
     6841 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6901 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6961 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7021 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7081 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7141 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7201 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     7261 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7321 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7381 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc
     7441 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     7501 ggctagaagc acaagaggag gaggaggtgg gtttttccag tcacacctca ggtaccttta
     7561 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga
     7621 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     7681 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     7741 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     7801 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     7861 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     7921 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     7981 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     8041 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     8101 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8161 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8221 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     8281 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta
     8341 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     8401 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8461 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8521 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8581 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8641 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8701 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8761 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8821 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8881 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8941 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9001 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9061 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9121 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9181 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9241 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9301 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9361 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9421 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9481 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9541 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9601 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9661 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9721 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9781 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9841 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9901 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9961 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10021 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10081 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10141 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10201 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10261 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10321 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10381 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10441 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10501 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg
    10561 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    10621 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    10681 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    10741 cctattacca ctgccaatta cctagtggtt tcatttactc taaacctgtg attcctctga
    10801 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt
    10861 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
