LOCUS       sequence-114429-       10461 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mKate2^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 10461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD285
FEATURES             Location/Qualifiers
     source          1..10461
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             661..966
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             1887..1907
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(1925..1958)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1968..1997
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3814..4137)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6055..6079
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6082..7107
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6082..7107
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7108..7132)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7485..7518)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7537..7560
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7564..7587
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7591..7614
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(7662..7967)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8240..8256)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8240..8256)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8253..8275)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8264..8280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8288..8294,8295..8312,8313..8318))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8333..8354
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8471..8488)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8642..9230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8722..8741)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9401..10192,10193..10261))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10024..10043
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10262..10366)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10434..10452
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
      421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
      541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
      661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
     1021 gagcctcagg agcatcgatg gtctccgagc tcattaaaga aaacatgcat atgaagctct
     1081 acatggaggg aaccgtcaac aaccaccact tcaagtgcac ctccgaggga gagggaaagc
     1141 catacgaggg aacccaaacc atgcgtatca aggccgtcga gggaggacca ctcccattcg
     1201 ccttcgacat cctcgccacc tccttcatgt acggatccaa ggtaagttta aacatatata
     1261 tactaactaa ccctgattat ttaaattttc agaccttcat caaccacacc caaggaatcc
     1321 cagacttctt caagcaatcc ttcccagagg gattcacctg ggagcgtgtc accacctacg
     1381 aggacggagg agtcctcacc gccacccaag acacctccct ccaagacgga tgcctcatct
     1441 acaacgtcaa ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     1501 agatccgtgg agtcaacttc ccatccaacg gaccagtcat gcaaaagaag accctcggat
     1561 gggaggcctc caccgagacc ctctacccag ccgacggagg acttgaggga cgtgccgaca
     1621 tggccctcaa gctcgtcgga ggaggacacc tcatctgcaa cctcaaggta agtttaaaca
     1681 tgattttact aactaactaa tctgatttaa attttcagac cacctaccgt tccaagaagc
     1741 cagccaagaa cctcaagatg ccaggagtct actacgtcga ccgtcgtctt gagcgtatca
     1801 aggaggccga caaggagacc tacgtcgagc aacacgaggt cgccgtcgcc cgttactgcg
     1861 acctcccatc caagctcgga caccgtgaga atctgtactt tcaatccgga aaggtaagtt
     1921 taaaataact tcgtatagca tacattatac gaagttattt tcagggagag caaaagctta
     1981 tctccgagga ggacctctaa ggatgatcga cgccaacgtc gttgaatttt caaattttaa
     2041 atactgaata tttgtttttt ttcctattat ttatttattc tctttgtgtt ttttttcttg
     2101 ctttctaaaa aattaattca atccaaatct aaacattttt ttttctcttt ccgtctccca
     2161 attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt
     2221 catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga
     2281 aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt
     2341 aaaagaaaac ataaaaatat atagaggagg aaggaaacta gctgccctcg attgtctatc
     2401 ttccattctc accaagtttc aacaacttta ttcagtcatt cttctctggt tcatttccca
     2461 accaatcttc ccgaataaag cccatctgtc ctttcggcca cattcctcac ctacatccgt
     2521 actccttatc tcccgttctt atccagtctt ttatcgtctc tgcgtctcgc acactgtctc
     2581 gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact tgcggaaaga tttcctaata
     2641 aaaggagacg aatccggtag gaaatcgaca agtctctgct tctcgaagac taggtgagtt
     2701 acatttcttt ggccgaatta ttttgaaatc ctaaatttga taatttcaga gatgtctgta
     2761 aaacttgcgt gttatgtgac ggcttcggtc actgtcgcca ctcttatggt atgcttcatg
     2821 accatgtcaa ccatctactc ggaggttgat ggattcagag agaagcttga taccgagatg
     2881 aatgtgttca gagtgagtta aattatattt tgaattttaa ataattttta attttctagc
     2941 aatctaccaa tggattgtgg aaggacatag ttgtcatcgg aagatctagc aagcgtgtcc
     3001 gttgtcaata tgaagagacc aacgctaccc caactccaca tgctgatgga tccccatctg
     3061 ctccaccagg tcaaccacca gcagttccac cagtcttcaa ccagccaaag actccaaatg
     3121 gagccaatgg aaatggacca acctgcaact gcaatgctga taacaagtgc ccagctggac
     3181 catccggacc aaagggagtt ccaggagttc caggactcga cggagttcca ggacttgacg
     3241 gtgttccagg agttggagct gatgatatcg ctccacaacg cgagtctgtc ggatgcttca
     3301 cttgcccaca aggaccagtt ggaccaccag gagctcttgg aagaccagga ccacgtggac
     3361 ttccaggacc aagaggacaa aatggaaacc caggaagaga tggacaacca ggacatccag
     3421 gagagcaagg atcatccggt caaatcggaa agatcggaga gccaggacca ccaggagaga
     3481 agggacgcga cgccgagcat ccaatcggaa gaccaggacc aaagggacca agaggagatc
     3541 aaggaccaac aggaccagct ggacagaacg gtcttcacgg accaccagga gagccaggaa
     3601 ccgttggacc agaaggacca tctggaaagc aaggacgtca aggaccagac ggaacccagg
     3661 gagagactgg accagacgga agaccaggaa aggatgccga gtactgccag tgcccagaca
     3721 agtctccacc atcagaggct gtcaacgcca accgtggata cagaaatatc taaattgttg
     3781 gtgttttcta ataaaatatt tgagattcag tgagactttt ttcttggcgg cacaataaag
     3841 tttcctcttt ttgttgggtc actcaacgaa cacaatacaa atatttgagc gctcgcattt
     3901 ttctcaacct ttcgggttta aagcacttca aaatggaatt gaagagagat tgtgaaagta
     3961 catcagacag aatcacattt tgatagcatt cacttcactc agatgcaaac ttttcagtga
     4021 ctataaaaag tcataaaata ataacattaa aaaagcaaat aaattagcga gagaattttt
     4081 tgacaaaaag aaagaagagt gatagagaag aagggaatgc ttgaaaggat tttgcattta
     4141 tcttaatcac cgtcttcgag aagacgaacc atggcgccag tttcagaatc caagtttcta
     4201 atatagttca tcacaatgtt gacgttggtc caacctccgg cttgcataat ctctgggatg
     4261 gaaactccag cgcgagccat gtcacgggca gctccgacac gggcggaatg tccagaccaa
     4321 gccaagtaac gttgtccgga atcatccttg gctccgtaga tgagacggtg ggtagcttcg
     4381 aagattcctg aaaatttatt ttcagcttat tcttagctaa tatgttgttt aaacttacct
     4441 tcaagagctc gagtcgagag ttgtgatgtt gcactcgggg cagcaactcc gttcttgcga
     4501 acacgacaga acaggtagtt gtttggatca tcggcgactc cggaaaccga aatccagcgc
     4561 tcaacgagct tggtaactcc aagggagagc gctttttcga ctccggcagt cgagaccagc
     4621 gtttttgtac gtccgatgtg gatgagcatt cttcctccgt ctgtgcgact aatatccttg
     4681 acgcgaatgc gggcaatttc ggcgatacgg agaagtgtgt tgtaagcaat tccgaggaag
     4741 gcgaggtttc ggatgtcctg gcaacgatcg gaattttcca taagagaacg gacctgatcg
     4801 aagtcggttc tgaaaatttc gtcaagtctt aggattgaat acattaatat ttaaacttac
     4861 ctttcgaagg cgagggcttg cttggctctc tctccagcat caacattttc cttgcggata
     4921 cgtctcatta cgagagatac ggcattagaa tctgatgggc gtggaagtcc agatcggcga
     4981 tggagcatat tgagctgtcc gagatgttgt tggatcgtct tcaccgccaa tccacgagct
     5041 tggaggtaga gcaagtagtc acgaacgtct tctggctcgg ctgggaacca cttgcggttg
     5101 ttaagcttgc accaggcagc ccaagaacgg cacactgaga gaagcatctt ccaggtgtgc
     5161 tcggagaagg cttgtctgtc acggaacata tccatgaggt tcttgcgaac ttcatcactt
     5221 gtcgcgtcaa ctggaagtgc tggaagattt tgatgaacag tgagaagatt tgacatattt
     5281 tcgaagtttt ttagatgcac tagaacaaag cgtgttggct tcctctgagc ccgctttcct
     5341 tatatacccg cattctgcag ccttacagaa tgttctagaa ggtcctagat gcattcgttt
     5401 gaaaatactc ccggtgggtg caaagagacg cagacggaaa atgtatctgg gtctctttat
     5461 tgtgtacact acttttccat gtaccgaatg tgagtcgccc tccttttgca acaagcagct
     5521 cgaatgttct agaaaaaggt ggaaaatagt ataaataccg ttgaaaataa ataccgaaca
     5581 acatttgctc taattgtgaa attagaaatc ttcaaactat aatcatgtca ctttaccact
     5641 atttccgtcc agctcaacgt tccgtttttg gtgatcattt ttgctttcgt cgtaaatcta
     5701 cacacgcgtc tcttccgtgc gagagtccaa gccagcagcc aaattcgttg actgagtatt
     5761 caacgtttat acgttgtcgg caacgagaaa taggaaaatg catcgggaaa tgttcttttt
     5821 tcgatttttt ccaaggtttt gacaaatttt accacgaatt ttgctatgtt ttcaattaaa
     5881 aaatatgtta ttcaactgtt tctatgagga aaataaggct ttgcatgtaa ttttcttatt
     5941 cagcataatt tttaattaat ttgaattttc tgtcctaacg tttattttgt tttcttggtt
     6001 atgactgatc tgaaattaat ttttgaattt taaggtaata tgtcaggcgg tgccgcaagt
     6061 ttgtacaaaa aagcaggctc catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     6121 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     6181 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     6241 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     6301 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
     6361 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     6421 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     6481 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     6541 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     6601 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     6661 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     6721 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     6781 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     6841 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     6901 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     6961 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     7021 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     7081 agcactcgtc cgagggcaaa ggaatagacc cagctttctt gtacaaagtg ggtccaatta
     7141 ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta
     7201 tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg
     7261 aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct
     7321 ccctcccccc attaataata attctatccc aaaatctaca caatgttctg tgtacacttc
     7381 ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa
     7441 taccttatca tatgttacgt ttcagtttat gaccgcaatt tttaataact tcgtatagca
     7501 tacattatac gaagttattt tcagggagcc ggatctgatt ataaagacga tgacgataag
     7561 cgtgactaca aggacgacga cgacaagcgt gattacaagg atgacgatga caagagacta
     7621 gttcgacctg cagactggct gtgtataagg gagcctgaca tttatattcc ccagaacatc
     7681 aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg
     7741 ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg
     7801 atatgcacca ccgggtaaag ttcacggggg actttatctg acagcagacg tgcactggcc
     7861 agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac
     7921 agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc accagcccct
     7981 gttctcgtcg gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc
     8041 ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt
     8101 gcttaccgaa ccggagatat tgacatcata tatgccttga gcaactgata gctgtcgctg
     8161 tcaactgtca ctgtaatacg ctgcttcata gcatacctct ttttgacata cttcgggttc
     8221 ctaggaaatc gataacatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8281 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8341 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8401 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8461 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8521 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8581 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8641 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8701 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8761 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8821 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8881 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8941 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9001 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9061 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     9121 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9181 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9241 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9301 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9361 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9421 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9481 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9541 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9601 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9661 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9721 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9781 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9841 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9901 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9961 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10021 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10081 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10141 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10201 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10261 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10321 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10381 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    10441 gtatcacgag gccctttcgt c
//
