LOCUS       sequence-114447-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter construct containing a 742 bp promoter region of the Ihh 
            gene in front of a luciferase gene for in vitro DNA transfection 
            assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Wang W, Lian N, Li L, Moss HE, Wang W, Perrien DS, Elefteriou F, 
            Yang X
  TITLE     Atf4 regulates chondrocyte proliferation and differentiation during 
            endochondral ossification by activating Ihh transcription.
  JOURNAL   Development. 2009 Dec;136(24):4143-53. doi: 10.1242/dev.043281. Epub
            2009 Nov 11.
  PUBMED    19906842
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.043281"; journalName: "Development"; date: "2009-12"; 
            volume: "136"; issue: "24"; pages: "4143-53"
COMMENT     Sequence Label: pIhh742bp-Luc
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     98..114
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     98..114
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat tttaccaaca gtaccggaat
       61 gccaagcttg tcgactagtc cgcggtaccg cggccgctct agaactagtg gatcccctta
      121 ctagtcttgg ggactccatg cgggggcgcc atgggcagcg cgacgcggct caaggccgac
      181 gggactcagg tgcggggcgg gggcccgggg ccctggctgg tggcggcgtg ctgtccccct
      241 cggcgcctcg actctgagct gcccggctcg ccggccgcca ataaataggc cggcccgttt
      301 gttttggcaa cgcggcgacg gcggggggcg gcgggcggcg gggctgaggg ccggcgggct
      361 gggctgggct ggcccggccg gcggactgaa gggacccgcg gttagcaccc cggcccggcg
      421 gtcaggcggc tcgggcagag cgggagctgc tgccgtctgc ggcgcagccc ggggtcgagt
      481 gagaggggaa atggaagaga tccgggctcg ggacccgcgc agaccgcacc ctatccatgt
      541 ccctgcctcc tgcgcgctcg acagcgaacc tgcagcaagg gcagcacagc ctcctccccc
      601 tccggcgggc ggccccaagg ctagccagcc ctgtcccgcg cgctcctcag ccgcgcgtcc
      661 acccgcccgc tgccctttgc gccccctgcg ctgcctgcga ttgtcctcct gacgggcctc
      721 accccaccct ggtctctcct tcccgttcct tgcggctcct gggaagcccc accaggcgca
      781 aatctcctgg acaggagatg ccacccccac ggagaccgca acccacgtc
//
