LOCUS       sequence-114452-        5352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Glucarate biosensor. High copy plasmid that expresses GFP 
            proportional to glucarate concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-H-cdaR
FEATURES             Location/Qualifiers
     source          1..5352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(59..127,128..919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(277..296)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1090..1678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1579..1598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(1767..1784)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1937..2023
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2115..2142
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      5272..5319
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
       61 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      121 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      181 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      241 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      301 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      361 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      421 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      481 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      541 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      601 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      661 ccggcaacaa ttgatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      721 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg
      781 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
      841 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
      901 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
      961 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1021 caaaatccct taacgtgggt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1081 aggatcatct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1141 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1201 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1261 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1321 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1381 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1441 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1501 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1561 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1621 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1681 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcaggctgt
     1741 tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     1801 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     1861 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
     1921 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1981 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     2041 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     2101 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactat
     2161 gctgttgatt gacgccagtg agaacccgga accggaaacg gaatcaaatc cgtgggtcga
     2221 acagtggggc acgctgttgt cctgatatgt tcagcgagcg gtaaatgtcg ttttagcggt
     2281 gctgaatcga atcttttcag gcaaatgcca gtaaaaactg cttcatagcg cggattttta
     2341 ctggcgtttg cctggagtca agcgatccat ttcatactct tctttatttc ttcgttttaa
     2401 cccttccttt cttgttcttg ttttcatttc cgtgaagtgg attccaccgt ccagggctaa
     2461 tgccaaaatc gggcctcatt gaacgcatta atgttgtgtt gttgcacggt gagccgctat
     2521 ggcgcgcttt ttatactgct attgccagat ataaacacgc gccgtattcg gcgaacgacc
     2581 tataaaaacg gcaaaaaaca ccctacgtca cctctgattt cctggcgatg tcgcagtcca
     2641 gagtgagcgt ggctaacgcg aattttcagg agtgcaacat ttaactttaa gaaggagata
     2701 tacatatgcg tataggtgaa gaactgttca ccggtgttgt tccgatcctg gttgaactgg
     2761 acggtgacgt taacggtcac aaattctctg ttcgtggtga aggtgaaggt gacgctacca
     2821 acggtaaact gaccctgaaa ttcatctgca ccaccggtaa actgccggtt ccgtggccga
     2881 ccctggttac caccctgacc tacggtgttc agtgcttcgc tcgttacccg gaccacatga
     2941 aacagcacga cttcttcaaa tctgctatgc cggaaggtta cgttcaggaa cgtaccatct
     3001 ctttcaaaga cgacggtacc tacaaaaccc gtgctgaagt taaattcgaa ggtgacaccc
     3061 tggttaaccg tatcgaactg aaaggtatcg acttcaaaga agacggtaac atcctgggtc
     3121 acaaactgga atacaacttc aactctcaca acgtttacat caccgctgac aaacagaaaa
     3181 acggtatcaa agctaacttc aaaatccgtc acaacgttga agacggttct gttcagctgg
     3241 ctgaccacta ccagcagaac accccgatcg gtgacggtcc ggttctgctg ccggacaacc
     3301 actacctgtc tacccagtct gttctgtcta aagacccgaa cgaaaaacgt gaccacatgg
     3361 ttctgctgga attcgttacc gctgctggta tcacccacgg tatggacgaa ctgtacaaag
     3421 atgcatgcca gttctagcat aaccctaatg agtgagctaa cttacattaa ttgcgttgcg
     3481 ccttaattaa cggcactcct cagcaaatat aatgaccctc ttgataaccc aagagggcat
     3541 tttttaatgc ccatggcgtt taccaaactg accctgggct tactgcgcga cggtaagcag
     3601 gttaacgtga acctggaact gcagcagagc agccagaatc aggttgattc cagctccatc
     3661 ttcaacggca ttgaaggcgc tgagatgagc aacaaaggca aagatcaggg cgtggtagtg
     3721 aacaacgtga aaacgggcac tccggctgcg cagatcggcc tgaagaaagg tgatgtgatt
     3781 attggcgcga accagcaggc agtgaaaaac atcgctgaac tgcgtaaagt tctcgacagc
     3841 aaaccgtctg tgctggcact caacattcag cgcggcgaca gcaccatcta cctgttaatg
     3901 cagtaatctc cctcaacccc ttcctgaaaa cgggaagggg ttctccttac aatctgtgaa
     3961 cttcaccaca actccataca tcttcatcat cctttaggca tttgcacaat gccgtacgtt
     4021 acgtacttcc ttatgctaag ccgtgcataa cggaggactt atggctggct ggcatcttga
     4081 taccaaaatg gcgcaggata tcgtggcacg taccatgcgc atcatcgata ccaatatcaa
     4141 cgtaatggat gcccgtgggc gaattatcgg cagcggcgat cgtgagcgta ttggtgaatt
     4201 gcacgaaggt gcattgctgg tactttcaca gggacgagtc gtcgatatcg atgacgcggt
     4261 agcacgtcat ctgcacggtg tgcggcaggg gattaatcta ccgttacggc tggaaggtga
     4321 aattgtcggc gtaattggcc tgacaggtga accagagaat ctgcgtaaat atggcgaact
     4381 ggtctgcatg acggctgaaa tgatgctgga acagtcgcgg ttgatgcact tgttggcgca
     4441 ggatagccgt ttgcgggaag aactggtgat gaacctgatt caggcagagg agaatactcc
     4501 cgcacttact gaatgggcgc aacggctggg gatcgatctc aatcaaccgc gagtggtggc
     4561 tattgttgag gtcgacagcg gtcagcttgg cgtggacagc gcaatggcgg agttacaaca
     4621 actgcaaaac gcgctgacta cgcccgagcg taataatctg gtggcgattg tctcgctaac
     4681 cgaaatggtg gtgttgaaac cggcgttgaa ctcttttggg cgctgggatg cagaagatca
     4741 tcgtaagcga gttgaacaac tgattacccg catgaaagag tacggccagc tgcgttttcg
     4801 cgtttcactg ggcaactatt ttaccggtcc tagcagtatt gcccgatcct atcgtacggc
     4861 gaaaacgacg atggtggtgg gtaaacagcg gatgccagaa agtcgctgct atttttatca
     4921 ggatctgatg ttacctgtgt tactcgacag tttgcgtggc gactggcagg ccaacgaact
     4981 ggcgcgaccg ctggcgcggc tgaaaacgat ggacaataac ggcttgctgc gacgaacgct
     5041 ggcggcgtgg tttcgccaca atgtgcaacc gctggcaacg tcaaaggcgt tgtttattca
     5101 tcgtaatacc ctggagtatc ggcttaatcg tatatcggaa ctgaccgggc ttgatttggg
     5161 caattttgat gacaggttgc tgctgtatgt ggcgttacaa ctggatgaag agcggtagta
     5221 agcgactaaa aaattgaatg taggaaacca acatgccagt tcgagcaata actagcataa
     5281 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaacctcag gcgagaagca
     5341 cacggtcaca ct
//
