LOCUS       sequence-11446-e       12166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12166)
  AUTHORS   Boy AL, Zhai Z, Habring-Muller A, Kussler-Schneider Y, Kaspar P, 
            Lohmann I.
  TITLE     Vectors for efficient and high-throughput construction of 
            fluorescent Drosophila reporters using the PhiC31 site-specific 
            integration system.
  JOURNAL   Genesis. 2010 Apr 22. [Epub ahead of print]
  PUBMED    20506180
REFERENCE   2  (bases 1 to 12166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2010 Apr 22. [Epub ahead of print]"
COMMENT     Sequence Label: pHPdesteGFP
FEATURES             Location/Qualifiers
     source          1..12166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    63..187
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(212..217,218..235,236..242)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             296..955
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(362..381)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1297..1602
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1481..1500
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1643..1767)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1974..1983
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1980..1982,1983..1985,1986..2699)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1980..1982,1983..1985,1986..2696)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2025..2046)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2286..2305)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2633..2654
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2826..2947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2863..2882)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2917..2936
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    2998..3067
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    3268..3653
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     gene            3654..7780
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(3663..3681)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     misc_feature    join(7791..8345,8346..8376)
                     /label=P element 5' end
     rep_origin      complement(8611..9199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8691..8710)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9370..10161,10162..10230))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9993..10012
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10231..10335)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10403..10421
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10459..10481)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10581..10600
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(11137..11167,11168..11369)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    11768..12153
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
ORIGIN
        1 gcatgctgca gcagatctgg tctagagccc gggcgaattc gccggcgcgc cggtaccatc
       61 aaacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
      121 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
      181 catattggcg gccccattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
      241 tgtgtggatt ttgagttagg agccgtcgag attttcagga gctaaggaag ctaaaatgga
      301 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
      361 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
      421 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
      481 tcttgcccgc ctgatgaatg ctcatccgga gttccgtatg gcaatgaaag acggtgagct
      541 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
      601 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
      661 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
      721 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
      781 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
      841 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
      901 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg
      961 tggagccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     1021 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     1081 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     1141 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     1201 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     1261 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     1321 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     1381 ggccgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     1441 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     1501 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     1561 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     1621 cacagccagt ctgcacctcg accatagtga ctggatatgt tgtgttttac agtattatgt
     1681 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     1741 tcgttcagct ttcttgtaca aagtggttcg atctcgagga gcgccggagt ataaatagag
     1801 gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac gtcgctaagc
     1861 gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatctgca gccaagctga
     1921 tcctctaggg tacgcgctag agtcgagagg cctgtttaaa cgatccaccg gtcgccacca
     1981 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2041 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2101 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     2161 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     2221 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     2281 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     2341 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     2401 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     2461 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     2521 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2581 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     2641 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa
     2701 gcggccggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
     2761 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     2821 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     2881 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     2941 tatcttaagg cgtaaattgt aagcgttaat actagtgtcg acgatgtagg tcacggtctc
     3001 gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc
     3061 acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc
     3121 gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca cggtgagcac
     3181 gggacgtgcg acggcgtcgg cgggtgcgga tacgcggggc agcgtcagcg ggttctcgac
     3241 ggtcacggcg ggcatgtcga cactagtcac gtaataagtg tgcgttgaat ttattcgcaa
     3301 aaacattgca tattttcggc aaagtaaaat tttgttgcat accttatcaa aaaataagtg
     3361 ctgcatactt tttagagaaa ccaaataatt ttttattgca tacccgtttt taataaaata
     3421 cattgcatac cctcttttaa taaaaaatat tgcatacttt gacgaaacaa attttcgttg
     3481 catacccaat aaaagattat tatattgcat acccgttttt aataaaatac attgcatacc
     3541 ctcttttaat aaaaaatatt gcatacgttg acgaaacaaa ttttcgttgc atacccaata
     3601 aaagattatt atattgcata ccttttcttg ccataccatt tagccgatca attctagtat
     3661 gtatgtaagt taataaaacc catttttgcg gaaagtagat aaaaaaaaca tttttttttt
     3721 ttactgcact ggatatcatt gaacttatct gatcagtttt aaatttactt cgatccaagg
     3781 gtatttgatg taccaggttc tttcgattac ctctcactca aaatgacatt ccactcaaag
     3841 tcagcgctgt ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt cgccgctgcg
     3901 tccgctatct ctttcgccac cgtttgtagc gttacctagc gtcaatgtcc gccttcagtt
     3961 gcactttgtc agcggtttcg tgacgaagct ccaagcggtt tacgccatca attaaacaca
     4021 aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt tgttttttga gtgattgggg
     4081 tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg caatgggcca
     4141 agaggatcag gagctattaa ttcgcggagg cagcaaacac ccatctgccg agcatctgaa
     4201 caatgtgagt agtacatgtg catacatctt aagttcactt gatctatagg aactgcgatt
     4261 gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac aaaaatccca aaccgcaatc
     4321 gcacaaacaa atagtgacac gaaacagatt attctggtag ctgtgctcgc tatataagac
     4381 aatttttaag atcatatcat gatcaagaca tctaaaggca ttcattttcg actacattct
     4441 tttttacaaa aaatataaca accagatatt ttaagctgat cctagatgca caaaaaataa
     4501 ataaaagtat aaacctactt cgtaggatac ttcgttttgt tcggggttag atgagcataa
     4561 cgcttgtagt tgatatttga gatcccctat cattgcaggg tgacagcgga cgcttcgcag
     4621 agctgcatta accagggctt cgggcaggcc aaaaactacg gcacgctcct gccacccagt
     4681 ccgccggagg actccggttc agggagcggc caactagccg agaacctcac ctatgcctgg
     4741 cacaatatgg acatctttgg ggcggtcaat cagccgggct ccggatggcg gcagctggtc
     4801 aaccggacac gcggactatt ctgcaacgag cgacacatac cggcgcccag gaaacatttg
     4861 ctcaagaacg gtgagtttct attcgcagtc ggctgatctg tgtgaaatct taataaaggg
     4921 tccaattacc aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa cttttggccg
     4981 tgatgggcag ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc
     5041 cgcagggcat ccaagtatcg ccatccggga tgcgactgct caatggccaa cctgtggacg
     5101 ccaaggagat gcaggccagg tgcgcctatg tccagcagga tgacctcttt atcggctccc
     5161 taacggccag ggaacacctg attttccagg ccatggtgcg gatgccacga catctgacct
     5221 atcggcagcg agtggcccgc gtggatcagg tgatccagga gctttcgctc agcaaatgtc
     5281 agcacacgat catcggtgtg cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc
     5341 gtctggcatt cgcctccgag gcactaaccg atccgccgct tctgatctgc gatgagccca
     5401 cctccggact ggactcattt accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc
     5461 agaagggcaa gaccgtcatc ctgaccattc atcagccgtc ttccgagctg tttgagctct
     5521 ttgacaagat ccttctgatg gccgagggca gggtagcttt cttgggcact cccagcgaag
     5581 ccgtcgactt cttttcctag tgagttcgat gtgtttatta agggtatcta gcattacatt
     5641 acatctcaac tcctatccag cgtgggtgcc cagtgtccta ccaactacaa tccggcggac
     5701 ttttacgtac aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc
     5761 gccaagatat gcgacaattt tgctattagc aaagtagccc gggatatgga gcagttgttg
     5821 gccaccaaaa atttggagaa gccactggag cagccggaga atgggtacac ctacaaggcc
     5881 acctggttca tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa
     5941 ccactcctcg taaaagtgcg acttattcag acaacggtga gtggttccag tggaaacaaa
     6001 tgatataacg cttacaattc ttggaaacaa attcgctaga ttttagttag aattgcctga
     6061 ttccacaccc ttcttagttt ttttcaatga gatgtatagt ttatagtttt gcagaaaata
     6121 aataaatttc atttaactcg cgaacatgtt gaagatatga atattaatga gatgcgagta
     6181 acattttaat ttgcagatgg ttgccatctt gattggcctc atctttttgg gccaacaact
     6241 cacgcaagtg ggcgtgatga atatcaacgg agccatcttc ctcttcctga ccaacatgac
     6301 ctttcaaaac gtctttgcca cgataaatgt aagtcttgtt tagaatacat ttgcatatta
     6361 ataatttact aactttctaa tgaatcgatt cgatttaggt gttcacctca gagctgccag
     6421 tttttatgag ggaggcccga agtcgacttt atcgctgtga cacatacttt ctgggcaaaa
     6481 cgattgccga attaccgctt tttctcacag tgccactggt cttcacggcg attgcctatc
     6541 cgatgatcgg actgcgggcc ggagtgctgc acttcttcaa ctgcctggcg ctggtcactc
     6601 tggtggccaa tgtgtcaacg tccttcggat atctaatatc ctgcgccagc tcctcgacct
     6661 cgatggcgct gtctgtgggt ccgccggtta tcataccatt cctgctcttt ggcggcttct
     6721 tcttgaactc gggctcggtg ccagtatacc tcaaatggtt gtcgtacctc tcatggttcc
     6781 gttacgccaa cgagggtctg ctgattaacc aatgggcgga cgtggagccg ggcgaaatta
     6841 gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa ggtcatcctg gagacgctta
     6901 acttctccgc cgccgatctg ccgctggact acgtgggtct ggccattctc atcgtgagct
     6961 tccgggtgct cgcatatctg gctctaagac ttcgggcccg acgcaaggag tagccgacat
     7021 atatccgaaa taactgcttg tttttttttt taccattatt accatcgtgt ttactgttta
     7081 ttgccccctc aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta
     7141 tagaatacaa gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta
     7201 accaaaagac ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa
     7261 caccgggata tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac
     7321 ctccaccaca ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca
     7381 cattcacctt ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggttc
     7441 gctcaaatgg ttccgagtgg ttcatttcgt ctcaatagaa attagtaata aatatttgta
     7501 tgtacaattt atttgctcca atatatttgt atatatttcc ctcacagcta tatttattct
     7561 aatttaatat tatgactttt taaggtaatt ttttgtgacc tgttcggagt gattagcgtt
     7621 acaatttgaa ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa
     7681 atggtgggca taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat
     7741 acatttaatt tagaaaatgc ttggatttca ctggaactag aattaattcg gctgctgctc
     7801 taaacgacgc atttcgtact ccaaagtacg aattttttcc ctcaagctct tattttcatt
     7861 aaacaatgaa caggacctaa cgcacagtca cgttattgtt tacataaatg atttttttta
     7921 ctattcaaac ttactctgtt tgtgtactcc cactggtata gccttctttt atcttttctg
     7981 gttcaggctc tatcacttta ctaggtacgg catctgcgtt gagtcgcctc cttttaaatg
     8041 tctgaccttt tgcaggtgca gccttccact gcgaatcatt aaagtgggta tcacaaattt
     8101 gggagttttc accaaggctg cacccaaggc tctgctccca caattttctc ttaatagcac
     8161 acttcggcac gtgaattaat tttactccag tcacagcttt gcagcaaaat ttgcaatatt
     8221 tcattttttt ttattccacg taagggttaa tgttttcaaa aaaaaattcg tccgcacaca
     8281 acctttcctc tcaacaagca aacgtgcact gaatttaagt gtatacttcg gtaagcttcg
     8341 gctatcgacg ggaccacctt atgttatttc atcatgggcc agacccacgt agtccagcgg
     8401 cagatcggcg gcggagaagt taagcgtctc caggatgacc ttgcccgaac tggggcacgt
     8461 ggtgttcgac gatgtgcagc taatttcgcc cggctccacg tccgcccatt ggttaatcag
     8521 cagaccctcg ttggcgtaac ggaaccatga gaggtacgac aaccatttga ggtatactgg
     8581 caccgagccc gagttcaaga agaaggcgtt tttccatagg ctccgccccc ctgacgagca
     8641 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8701 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8761 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
     8821 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8881 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8941 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9001 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     9061 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9121 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9181 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9241 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9301 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9361 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9421 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9481 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9541 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9601 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9661 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9721 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9781 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9841 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9901 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9961 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
    10021 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10081 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10141 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10201 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10261 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10321 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    10381 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    10441 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    10501 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    10561 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    10621 gtgtgaaata ccgcaccgaa tcgcgcggaa ctaacgacag tcgctccaag gtcgtcgaac
    10681 aaaaggtgaa tgtgttgcgg agagcgggtg ggagacagcg aaagagcaac tacgaaacgt
    10741 ggtgtggtgg aggtgaatta tgaagagggc gcgcgatttg aaaagtatgt atataaaaaa
    10801 tatatcccgg tgttttatgt agcgataaac gagtttttga tgtaaggtat gcaggtgtgt
    10861 aagtcttttg gttagaagac aaatccaaag tctacttgtg gggatgttcg aaggggaaat
    10921 acttgtattc tataggtcat atcttgtttt tattggcaca aatataatta cattagcttt
    10981 ttgagggggc aataaacagt aaacacgatg gtaataatgg taaaaaaaaa aacaagcagt
    11041 tatttcggat atatgtcggc tactccttgc gtcgggcccg aagtcttaga gccagatatg
    11101 cgagcacccg gaagctcacg atgagaatgg ccagaccatg atgaaataac ataaggtggt
    11161 cccgtcggca agagacatcc acttaacgta tgcttgcaat aagtgcgagt gaaaggaata
    11221 gtattctgag tgtcgtattg agtctgagtg agacagcgat atgattgttg attaaccctt
    11281 agcatgtccg tggggtttga attaactcat aatattaatt agacgaaatt atttttaaag
    11341 ttttattttt aataatttgc gagtacgcaa agcttcctag gtcaagaccg acctgtccgg
    11401 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
    11461 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
    11521 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
    11581 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
    11641 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
    11701 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaagcttgg
    11761 gctgcatcac gtaataagtg tgcgttgaat ttattcgcaa aaacattgca tattttcggc
    11821 aaagtaaaat tttgttgcat accttatcaa aaaataagtg ctgcatactt tttagagaaa
    11881 ccaaataatt ttttattgca tacccgtttt taataaaata cattgcatac cctcttttaa
    11941 taaaaaatat tgcatacttt gacgaaacaa attttcgttg catacccaat aaaagattat
    12001 tatattgcat acccgttttt aataaaatac attgcatacc ctcttttaat aaaaaatatt
    12061 gcatacgttg acgaaacaaa ttttcgttgc atacccaata aaagattatt atattgcata
    12121 ccttttcttg ccataccatt tagccgatca attgtgctcg gcaaca
//
