LOCUS       sequence-11447-e       12184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Boy AL, Zhai Z, Habring-Muller A, Kussler-Schneider Y, Kaspar P, 
            Lohmann I.
  TITLE     Vectors for efficient and high-throughput construction of 
            fluorescent Drosophila reporters using the PhiC31 site-specific 
            integration system.
  JOURNAL   Genesis. 2010 Apr 22. [Epub ahead of print]
  PUBMED    20506180
REFERENCE   2  (bases 1 to 12184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2010 Apr 22. [Epub ahead of print]"
COMMENT     Sequence Label: pHPdestmCherry
FEATURES             Location/Qualifiers
     source          1..12184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    63..187
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(212..217,218..235,236..242)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             296..955
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(362..381)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1297..1602
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1481..1500
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1643..1767)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1974..1983
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1980..2687
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2125..2143)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2393..2412
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2691..2714
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    2844..2965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2881..2900)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2935..2954
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3016..3085
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    3286..3671
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     gene            3672..7798
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(3681..3699)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     misc_feature    join(7809..8363,8364..8394)
                     /label=P element 5' end
     rep_origin      complement(8629..9217)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8709..8728)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9388..10179,10180..10248))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10011..10030
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10249..10353)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10421..10439
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10477..10499)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10599..10618
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(11155..11185,11186..11387)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    11786..12171
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
ORIGIN
        1 gcatgctgca gcagatctgg tctagagccc gggcgaattc gccggcgcgc cggtaccatc
       61 aaacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
      121 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
      181 catattggcg gccccattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
      241 tgtgtggatt ttgagttagg agccgtcgag attttcagga gctaaggaag ctaaaatgga
      301 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
      361 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
      421 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
      481 tcttgcccgc ctgatgaatg ctcatccgga gttccgtatg gcaatgaaag acggtgagct
      541 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
      601 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
      661 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
      721 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
      781 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
      841 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
      901 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg
      961 tggagccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     1021 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     1081 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     1141 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     1201 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     1261 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     1321 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     1381 ggccgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     1441 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     1501 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     1561 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     1621 cacagccagt ctgcacctcg accatagtga ctggatatgt tgtgttttac agtattatgt
     1681 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     1741 tcgttcagct ttcttgtaca aagtggttcg atctcgagga gcgccggagt ataaatagag
     1801 gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac gtcgctaagc
     1861 gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatctgca gccaagctga
     1921 tcctctaggg tacgcgctag agtcgagagg cctgtttaaa cgatccaccg gtcgccacca
     1981 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     2041 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     2101 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     2161 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     2221 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     2281 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     2341 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     2401 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     2461 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     2521 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     2581 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     2641 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggcc tggagccacc
     2701 cccagttcga gaagtaaagc ggccggccgc gactctagat cataatcagc cataccacat
     2761 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2821 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2881 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2941 tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatac tagtgtcgac
     3001 gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc
     3061 gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga ggtggcggta
     3121 gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac cgctgggcgc
     3181 ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata cgcggggcag
     3241 cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca ctagtcacgt aataagtgtg
     3301 cgttgaattt attcgcaaaa acattgcata ttttcggcaa agtaaaattt tgttgcatac
     3361 cttatcaaaa aataagtgct gcatactttt tagagaaacc aaataatttt ttattgcata
     3421 cccgttttta ataaaataca ttgcataccc tcttttaata aaaaatattg catactttga
     3481 cgaaacaaat tttcgttgca tacccaataa aagattatta tattgcatac ccgtttttaa
     3541 taaaatacat tgcataccct cttttaataa aaaatattgc atacgttgac gaaacaaatt
     3601 ttcgttgcat acccaataaa agattattat attgcatacc ttttcttgcc ataccattta
     3661 gccgatcaat tctagtatgt atgtaagtta ataaaaccca tttttgcgga aagtagataa
     3721 aaaaaacatt tttttttttt actgcactgg atatcattga acttatctga tcagttttaa
     3781 atttacttcg atccaagggt atttgatgta ccaggttctt tcgattacct ctcactcaaa
     3841 atgacattcc actcaaagtc agcgctgttt gcctccttct ctgtccacag aaatatcgcc
     3901 gtctctttcg ccgctgcgtc cgctatctct ttcgccaccg tttgtagcgt tacctagcgt
     3961 caatgtccgc cttcagttgc actttgtcag cggtttcgtg acgaagctcc aagcggttta
     4021 cgccatcaat taaacacaaa gtgctgtgcc aaaactcctc tcgcttctta tttttgtttg
     4081 ttttttgagt gattggggtg gtgattggtt ttgggtgggt aagcagggga aagtgtgaaa
     4141 aatcccggca atgggccaag aggatcagga gctattaatt cgcggaggca gcaaacaccc
     4201 atctgccgag catctgaaca atgtgagtag tacatgtgca tacatcttaa gttcacttga
     4261 tctataggaa ctgcgattgc aacatcaaat tgtctgcggc gtgagaactg cgacccacaa
     4321 aaatcccaaa ccgcaatcgc acaaacaaat agtgacacga aacagattat tctggtagct
     4381 gtgctcgcta tataagacaa tttttaagat catatcatga tcaagacatc taaaggcatt
     4441 cattttcgac tacattcttt tttacaaaaa atataacaac cagatatttt aagctgatcc
     4501 tagatgcaca aaaaataaat aaaagtataa acctacttcg taggatactt cgttttgttc
     4561 ggggttagat gagcataacg cttgtagttg atatttgaga tcccctatca ttgcagggtg
     4621 acagcggacg cttcgcagag ctgcattaac cagggcttcg ggcaggccaa aaactacggc
     4681 acgctcctgc cacccagtcc gccggaggac tccggttcag ggagcggcca actagccgag
     4741 aacctcacct atgcctggca caatatggac atctttgggg cggtcaatca gccgggctcc
     4801 ggatggcggc agctggtcaa ccggacacgc ggactattct gcaacgagcg acacataccg
     4861 gcgcccagga aacatttgct caagaacggt gagtttctat tcgcagtcgg ctgatctgtg
     4921 tgaaatctta ataaagggtc caattaccaa tttgaaactc agtttgcggc gtggcctatc
     4981 cgggcgaact tttggccgtg atgggcagtt ccggtgccgg aaagacgacc ctgctgaatg
     5041 cccttgcctt tcgatcgccg cagggcatcc aagtatcgcc atccgggatg cgactgctca
     5101 atggccaacc tgtggacgcc aaggagatgc aggccaggtg cgcctatgtc cagcaggatg
     5161 acctctttat cggctcccta acggccaggg aacacctgat tttccaggcc atggtgcgga
     5221 tgccacgaca tctgacctat cggcagcgag tggcccgcgt ggatcaggtg atccaggagc
     5281 tttcgctcag caaatgtcag cacacgatca tcggtgtgcc cggcagggtg aaaggtctgt
     5341 ccggcggaga aaggaagcgt ctggcattcg cctccgaggc actaaccgat ccgccgcttc
     5401 tgatctgcga tgagcccacc tccggactgg actcatttac cgcccacagc gtcgtccagg
     5461 tgctgaagaa gctgtcgcag aagggcaaga ccgtcatcct gaccattcat cagccgtctt
     5521 ccgagctgtt tgagctcttt gacaagatcc ttctgatggc cgagggcagg gtagctttct
     5581 tgggcactcc cagcgaagcc gtcgacttct tttcctagtg agttcgatgt gtttattaag
     5641 ggtatctagc attacattac atctcaactc ctatccagcg tgggtgccca gtgtcctacc
     5701 aactacaatc cggcggactt ttacgtacag gtgttggccg ttgtgcccgg acgggagatc
     5761 gagtcccgtg atcggatcgc caagatatgc gacaattttg ctattagcaa agtagcccgg
     5821 gatatggagc agttgttggc caccaaaaat ttggagaagc cactggagca gccggagaat
     5881 gggtacacct acaaggccac ctggttcatg cagttccggg cggtcctgtg gcgatcctgg
     5941 ctgtcggtgc tcaaggaacc actcctcgta aaagtgcgac ttattcagac aacggtgagt
     6001 ggttccagtg gaaacaaatg atataacgct tacaattctt ggaaacaaat tcgctagatt
     6061 ttagttagaa ttgcctgatt ccacaccctt cttagttttt ttcaatgaga tgtatagttt
     6121 atagttttgc agaaaataaa taaatttcat ttaactcgcg aacatgttga agatatgaat
     6181 attaatgaga tgcgagtaac attttaattt gcagatggtt gccatcttga ttggcctcat
     6241 ctttttgggc caacaactca cgcaagtggg cgtgatgaat atcaacggag ccatcttcct
     6301 cttcctgacc aacatgacct ttcaaaacgt ctttgccacg ataaatgtaa gtcttgttta
     6361 gaatacattt gcatattaat aatttactaa ctttctaatg aatcgattcg atttaggtgt
     6421 tcacctcaga gctgccagtt tttatgaggg aggcccgaag tcgactttat cgctgtgaca
     6481 catactttct gggcaaaacg attgccgaat taccgctttt tctcacagtg ccactggtct
     6541 tcacggcgat tgcctatccg atgatcggac tgcgggccgg agtgctgcac ttcttcaact
     6601 gcctggcgct ggtcactctg gtggccaatg tgtcaacgtc cttcggatat ctaatatcct
     6661 gcgccagctc ctcgacctcg atggcgctgt ctgtgggtcc gccggttatc ataccattcc
     6721 tgctctttgg cggcttcttc ttgaactcgg gctcggtgcc agtatacctc aaatggttgt
     6781 cgtacctctc atggttccgt tacgccaacg agggtctgct gattaaccaa tgggcggacg
     6841 tggagccggg cgaaattagc tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg
     6901 tcatcctgga gacgcttaac ttctccgccg ccgatctgcc gctggactac gtgggtctgg
     6961 ccattctcat cgtgagcttc cgggtgctcg catatctggc tctaagactt cgggcccgac
     7021 gcaaggagta gccgacatat atccgaaata actgcttgtt ttttttttta ccattattac
     7081 catcgtgttt actgtttatt gccccctcaa aaagctaatg taattatatt tgtgccaata
     7141 aaaacaagat atgacctata gaatacaagt atttcccctt cgaacatccc cacaagtaga
     7201 ctttggattt gtcttctaac caaaagactt acacacctgc ataccttaca tcaaaaactc
     7261 gtttatcgct acataaaaca ccgggatata ttttttatat acatactttt caaatcgcgc
     7321 gccctcttca taattcacct ccaccacacc acgtttcgta gttgctcttt cgctgtctcc
     7381 cacccgctct ccgcaacaca ttcacctttt gttcgacgac cttggagcga ctgtcgttag
     7441 ttccgcgcga ttcggttcgc tcaaatggtt ccgagtggtt catttcgtct caatagaaat
     7501 tagtaataaa tatttgtatg tacaatttat ttgctccaat atatttgtat atatttccct
     7561 cacagctata tttattctaa tttaatatta tgacttttta aggtaatttt ttgtgacctg
     7621 ttcggagtga ttagcgttac aatttgaact gaaagtgaca tccagtgttt gttccttgtg
     7681 tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt ttatatatat caaaaataac
     7741 aactataata ataagaatac atttaattta gaaaatgctt ggatttcact ggaactagaa
     7801 ttaattcggc tgctgctcta aacgacgcat ttcgtactcc aaagtacgaa ttttttccct
     7861 caagctctta ttttcattaa acaatgaaca ggacctaacg cacagtcacg ttattgttta
     7921 cataaatgat tttttttact attcaaactt actctgtttg tgtactccca ctggtatagc
     7981 cttcttttat cttttctggt tcaggctcta tcactttact aggtacggca tctgcgttga
     8041 gtcgcctcct tttaaatgtc tgaccttttg caggtgcagc cttccactgc gaatcattaa
     8101 agtgggtatc acaaatttgg gagttttcac caaggctgca cccaaggctc tgctcccaca
     8161 attttctctt aatagcacac ttcggcacgt gaattaattt tactccagtc acagctttgc
     8221 agcaaaattt gcaatatttc attttttttt attccacgta agggttaatg ttttcaaaaa
     8281 aaaattcgtc cgcacacaac ctttcctctc aacaagcaaa cgtgcactga atttaagtgt
     8341 atacttcggt aagcttcggc tatcgacggg accaccttat gttatttcat catgggccag
     8401 acccacgtag tccagcggca gatcggcggc ggagaagtta agcgtctcca ggatgacctt
     8461 gcccgaactg gggcacgtgg tgttcgacga tgtgcagcta atttcgcccg gctccacgtc
     8521 cgcccattgg ttaatcagca gaccctcgtt ggcgtaacgg aaccatgaga ggtacgacaa
     8581 ccatttgagg tatactggca ccgagcccga gttcaagaag aaggcgtttt tccataggct
     8641 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8701 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8761 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8821 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8881 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8941 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9001 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9061 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9121 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9181 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9241 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9301 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9361 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9421 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9481 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9541 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9601 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9661 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9721 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9781 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9841 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9901 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9961 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc
    10021 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10081 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10141 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10201 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10261 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10321 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10381 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    10441 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    10501 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    10561 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    10621 gagagtgcac catatgcggt gtgaaatacc gcaccgaatc gcgcggaact aacgacagtc
    10681 gctccaaggt cgtcgaacaa aaggtgaatg tgttgcggag agcgggtggg agacagcgaa
    10741 agagcaacta cgaaacgtgg tgtggtggag gtgaattatg aagagggcgc gcgatttgaa
    10801 aagtatgtat ataaaaaata tatcccggtg ttttatgtag cgataaacga gtttttgatg
    10861 taaggtatgc aggtgtgtaa gtcttttggt tagaagacaa atccaaagtc tacttgtggg
    10921 gatgttcgaa ggggaaatac ttgtattcta taggtcatat cttgttttta ttggcacaaa
    10981 tataattaca ttagcttttt gagggggcaa taaacagtaa acacgatggt aataatggta
    11041 aaaaaaaaaa caagcagtta tttcggatat atgtcggcta ctccttgcgt cgggcccgaa
    11101 gtcttagagc cagatatgcg agcacccgga agctcacgat gagaatggcc agaccatgat
    11161 gaaataacat aaggtggtcc cgtcggcaag agacatccac ttaacgtatg cttgcaataa
    11221 gtgcgagtga aaggaatagt attctgagtg tcgtattgag tctgagtgag acagcgatat
    11281 gattgttgat taacccttag catgtccgtg gggtttgaat taactcataa tattaattag
    11341 acgaaattat ttttaaagtt ttatttttaa taatttgcga gtacgcaaag cttcctaggt
    11401 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
    11461 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
    11521 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
    11581 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
    11641 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
    11701 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
    11761 actgttcgcc aagcttgggc tgcatcacgt aataagtgtg cgttgaattt attcgcaaaa
    11821 acattgcata ttttcggcaa agtaaaattt tgttgcatac cttatcaaaa aataagtgct
    11881 gcatactttt tagagaaacc aaataatttt ttattgcata cccgttttta ataaaataca
    11941 ttgcataccc tcttttaata aaaaatattg catactttga cgaaacaaat tttcgttgca
    12001 tacccaataa aagattatta tattgcatac ccgtttttaa taaaatacat tgcataccct
    12061 cttttaataa aaaatattgc atacgttgac gaaacaaatt ttcgttgcat acccaataaa
    12121 agattattat attgcatacc ttttcttgcc ataccattta gccgatcaat tgtgctcggc
    12181 aaca
//
