LOCUS       sequence-11448-e       12196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Boy AL, Zhai Z, Habring-Muller A, Kussler-Schneider Y, Kaspar P, 
            Lohmann I.
  TITLE     Vectors for efficient and high-throughput construction of 
            fluorescent Drosophila reporters using the PhiC31 site-specific 
            integration system.
  JOURNAL   Genesis. 2010 Apr 22. [Epub ahead of print]
  PUBMED    20506180
REFERENCE   2  (bases 1 to 12196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2010 Apr 22. [Epub ahead of print]"
COMMENT     Sequence Label: pHPdestVenus
FEATURES             Location/Qualifiers
     source          1..12196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    63..187
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(212..217,218..235,236..242)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             296..955
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(362..381)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1297..1602
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1481..1500
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1643..1767)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1974..1983
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1980..1982,1983..1985,1986..2696)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2025..2046)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2286..2305)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2633..2654
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2703..2726
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    2856..2977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2893..2912)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2947..2966
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3201..3270
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    3298..3683
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     gene            3684..7810
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(3693..3711)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     misc_feature    join(7821..8375,8376..8406)
                     /label=P element 5' end
     rep_origin      complement(8641..9229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8721..8740)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9400..10191,10192..10260))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10023..10042
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10261..10365)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10433..10451
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10489..10511)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10611..10630
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(11167..11197,11198..11399)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    11798..12183
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
ORIGIN
        1 gcatgctgca gcagatctgg tctagagccc gggcgaattc gccggcgcgc cggtaccatc
       61 aaacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
      121 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
      181 catattggcg gccccattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
      241 tgtgtggatt ttgagttagg agccgtcgag attttcagga gctaaggaag ctaaaatgga
      301 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
      361 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
      421 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
      481 tcttgcccgc ctgatgaatg ctcatccgga gttccgtatg gcaatgaaag acggtgagct
      541 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
      601 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
      661 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
      721 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
      781 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
      841 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
      901 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg
      961 tggagccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     1021 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     1081 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     1141 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     1201 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     1261 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     1321 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     1381 ggccgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     1441 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     1501 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     1561 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     1621 cacagccagt ctgcacctcg accatagtga ctggatatgt tgtgttttac agtattatgt
     1681 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     1741 tcgttcagct ttcttgtaca aagtggttcg atctcgagga gcgccggagt ataaatagag
     1801 gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac gtcgctaagc
     1861 gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatctgca gccaagctga
     1921 tcctctaggg tacgcgctag agtcgagagg cctgtttaaa cgatccaccg gtcgccacca
     1981 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2041 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2101 gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     2161 tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
     2221 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     2281 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     2341 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     2401 agctggagta caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg
     2461 gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg
     2521 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2581 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     2641 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggag
     2701 cttggagcca tccccagttc gaaaagtaga gcggccggcc gcgactctag atcataatca
     2761 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2821 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     2881 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2941 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat
     3001 actagtgtcg acatgcccgc cgtgaccgtc gagaacccgc tgacgctgcc ccgcgtatcc
     3061 gcacccgccg acgccgtcgc acgtcccgtg ctcaccgtga ccaccgcgcc cagcggtttc
     3121 gagggcgagg gcttcccggt gcgccgcgcg ttcgccggga tcaactaccg ccacctcgac
     3181 ccgttcatca tgatggacca gatgggtgag gtggagtacg cgcccgggga gcccaagggc
     3241 acgccctggc acccgcaccg cggcttcgag accgtgacct acatcgtcga cactagtcac
     3301 gtaataagtg tgcgttgaat ttattcgcaa aaacattgca tattttcggc aaagtaaaat
     3361 tttgttgcat accttatcaa aaaataagtg ctgcatactt tttagagaaa ccaaataatt
     3421 ttttattgca tacccgtttt taataaaata cattgcatac cctcttttaa taaaaaatat
     3481 tgcatacttt gacgaaacaa attttcgttg catacccaat aaaagattat tatattgcat
     3541 acccgttttt aataaaatac attgcatacc ctcttttaat aaaaaatatt gcatacgttg
     3601 acgaaacaaa ttttcgttgc atacccaata aaagattatt atattgcata ccttttcttg
     3661 ccataccatt tagccgatca attctagtat gtatgtaagt taataaaacc catttttgcg
     3721 gaaagtagat aaaaaaaaca tttttttttt ttactgcact ggatatcatt gaacttatct
     3781 gatcagtttt aaatttactt cgatccaagg gtatttgatg taccaggttc tttcgattac
     3841 ctctcactca aaatgacatt ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac
     3901 agaaatatcg ccgtctcttt cgccgctgcg tccgctatct ctttcgccac cgtttgtagc
     3961 gttacctagc gtcaatgtcc gccttcagtt gcactttgtc agcggtttcg tgacgaagct
     4021 ccaagcggtt tacgccatca attaaacaca aagtgctgtg ccaaaactcc tctcgcttct
     4081 tatttttgtt tgttttttga gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg
     4141 gaaagtgtga aaaatcccgg caatgggcca agaggatcag gagctattaa ttcgcggagg
     4201 cagcaaacac ccatctgccg agcatctgaa caatgtgagt agtacatgtg catacatctt
     4261 aagttcactt gatctatagg aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac
     4321 tgcgacccac aaaaatccca aaccgcaatc gcacaaacaa atagtgacac gaaacagatt
     4381 attctggtag ctgtgctcgc tatataagac aatttttaag atcatatcat gatcaagaca
     4441 tctaaaggca ttcattttcg actacattct tttttacaaa aaatataaca accagatatt
     4501 ttaagctgat cctagatgca caaaaaataa ataaaagtat aaacctactt cgtaggatac
     4561 ttcgttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga gatcccctat
     4621 cattgcaggg tgacagcgga cgcttcgcag agctgcatta accagggctt cgggcaggcc
     4681 aaaaactacg gcacgctcct gccacccagt ccgccggagg actccggttc agggagcggc
     4741 caactagccg agaacctcac ctatgcctgg cacaatatgg acatctttgg ggcggtcaat
     4801 cagccgggct ccggatggcg gcagctggtc aaccggacac gcggactatt ctgcaacgag
     4861 cgacacatac cggcgcccag gaaacatttg ctcaagaacg gtgagtttct attcgcagtc
     4921 ggctgatctg tgtgaaatct taataaaggg tccaattacc aatttgaaac tcagtttgcg
     4981 gcgtggccta tccgggcgaa cttttggccg tgatgggcag ttccggtgcc ggaaagacga
     5041 ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg ccatccggga
     5101 tgcgactgct caatggccaa cctgtggacg ccaaggagat gcaggccagg tgcgcctatg
     5161 tccagcagga tgacctcttt atcggctccc taacggccag ggaacacctg attttccagg
     5221 ccatggtgcg gatgccacga catctgacct atcggcagcg agtggcccgc gtggatcagg
     5281 tgatccagga gctttcgctc agcaaatgtc agcacacgat catcggtgtg cccggcaggg
     5341 tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt cgcctccgag gcactaaccg
     5401 atccgccgct tctgatctgc gatgagccca cctccggact ggactcattt accgcccaca
     5461 gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc
     5521 atcagccgtc ttccgagctg tttgagctct ttgacaagat ccttctgatg gccgagggca
     5581 gggtagcttt cttgggcact cccagcgaag ccgtcgactt cttttcctag tgagttcgat
     5641 gtgtttatta agggtatcta gcattacatt acatctcaac tcctatccag cgtgggtgcc
     5701 cagtgtccta ccaactacaa tccggcggac ttttacgtac aggtgttggc cgttgtgccc
     5761 ggacgggaga tcgagtcccg tgatcggatc gccaagatat gcgacaattt tgctattagc
     5821 aaagtagccc gggatatgga gcagttgttg gccaccaaaa atttggagaa gccactggag
     5881 cagccggaga atgggtacac ctacaaggcc acctggttca tgcagttccg ggcggtcctg
     5941 tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg acttattcag
     6001 acaacggtga gtggttccag tggaaacaaa tgatataacg cttacaattc ttggaaacaa
     6061 attcgctaga ttttagttag aattgcctga ttccacaccc ttcttagttt ttttcaatga
     6121 gatgtatagt ttatagtttt gcagaaaata aataaatttc atttaactcg cgaacatgtt
     6181 gaagatatga atattaatga gatgcgagta acattttaat ttgcagatgg ttgccatctt
     6241 gattggcctc atctttttgg gccaacaact cacgcaagtg ggcgtgatga atatcaacgg
     6301 agccatcttc ctcttcctga ccaacatgac ctttcaaaac gtctttgcca cgataaatgt
     6361 aagtcttgtt tagaatacat ttgcatatta ataatttact aactttctaa tgaatcgatt
     6421 cgatttaggt gttcacctca gagctgccag tttttatgag ggaggcccga agtcgacttt
     6481 atcgctgtga cacatacttt ctgggcaaaa cgattgccga attaccgctt tttctcacag
     6541 tgccactggt cttcacggcg attgcctatc cgatgatcgg actgcgggcc ggagtgctgc
     6601 acttcttcaa ctgcctggcg ctggtcactc tggtggccaa tgtgtcaacg tccttcggat
     6661 atctaatatc ctgcgccagc tcctcgacct cgatggcgct gtctgtgggt ccgccggtta
     6721 tcataccatt cctgctcttt ggcggcttct tcttgaactc gggctcggtg ccagtatacc
     6781 tcaaatggtt gtcgtacctc tcatggttcc gttacgccaa cgagggtctg ctgattaacc
     6841 aatgggcgga cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc acgtgcccca
     6901 gttcgggcaa ggtcatcctg gagacgctta acttctccgc cgccgatctg ccgctggact
     6961 acgtgggtct ggccattctc atcgtgagct tccgggtgct cgcatatctg gctctaagac
     7021 ttcgggcccg acgcaaggag tagccgacat atatccgaaa taactgcttg tttttttttt
     7081 taccattatt accatcgtgt ttactgttta ttgccccctc aaaaagctaa tgtaattata
     7141 tttgtgccaa taaaaacaag atatgaccta tagaatacaa gtatttcccc ttcgaacatc
     7201 cccacaagta gactttggat ttgtcttcta accaaaagac ttacacacct gcatacctta
     7261 catcaaaaac tcgtttatcg ctacataaaa caccgggata tattttttat atacatactt
     7321 ttcaaatcgc gcgccctctt cataattcac ctccaccaca ccacgtttcg tagttgctct
     7381 ttcgctgtct cccacccgct ctccgcaaca cattcacctt ttgttcgacg accttggagc
     7441 gactgtcgtt agttccgcgc gattcggttc gctcaaatgg ttccgagtgg ttcatttcgt
     7501 ctcaatagaa attagtaata aatatttgta tgtacaattt atttgctcca atatatttgt
     7561 atatatttcc ctcacagcta tatttattct aatttaatat tatgactttt taaggtaatt
     7621 ttttgtgacc tgttcggagt gattagcgtt acaatttgaa ctgaaagtga catccagtgt
     7681 ttgttccttg tgtagatgca tctcaaaaaa atggtgggca taatagtgtt gtttatatat
     7741 atcaaaaata acaactataa taataagaat acatttaatt tagaaaatgc ttggatttca
     7801 ctggaactag aattaattcg gctgctgctc taaacgacgc atttcgtact ccaaagtacg
     7861 aattttttcc ctcaagctct tattttcatt aaacaatgaa caggacctaa cgcacagtca
     7921 cgttattgtt tacataaatg atttttttta ctattcaaac ttactctgtt tgtgtactcc
     7981 cactggtata gccttctttt atcttttctg gttcaggctc tatcacttta ctaggtacgg
     8041 catctgcgtt gagtcgcctc cttttaaatg tctgaccttt tgcaggtgca gccttccact
     8101 gcgaatcatt aaagtgggta tcacaaattt gggagttttc accaaggctg cacccaaggc
     8161 tctgctccca caattttctc ttaatagcac acttcggcac gtgaattaat tttactccag
     8221 tcacagcttt gcagcaaaat ttgcaatatt tcattttttt ttattccacg taagggttaa
     8281 tgttttcaaa aaaaaattcg tccgcacaca acctttcctc tcaacaagca aacgtgcact
     8341 gaatttaagt gtatacttcg gtaagcttcg gctatcgacg ggaccacctt atgttatttc
     8401 atcatgggcc agacccacgt agtccagcgg cagatcggcg gcggagaagt taagcgtctc
     8461 caggatgacc ttgcccgaac tggggcacgt ggtgttcgac gatgtgcagc taatttcgcc
     8521 cggctccacg tccgcccatt ggttaatcag cagaccctcg ttggcgtaac ggaaccatga
     8581 gaggtacgac aaccatttga ggtatactgg caccgagccc gagttcaaga agaaggcgtt
     8641 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8701 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8761 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8821 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8881 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8941 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9001 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9061 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     9121 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9181 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9241 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9301 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9361 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9421 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9481 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9541 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9601 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9661 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9721 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9781 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9841 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9901 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9961 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
    10021 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10081 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10141 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10201 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10261 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10321 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10381 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    10441 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    10501 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    10561 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    10621 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcaccgaa tcgcgcggaa
    10681 ctaacgacag tcgctccaag gtcgtcgaac aaaaggtgaa tgtgttgcgg agagcgggtg
    10741 ggagacagcg aaagagcaac tacgaaacgt ggtgtggtgg aggtgaatta tgaagagggc
    10801 gcgcgatttg aaaagtatgt atataaaaaa tatatcccgg tgttttatgt agcgataaac
    10861 gagtttttga tgtaaggtat gcaggtgtgt aagtcttttg gttagaagac aaatccaaag
    10921 tctacttgtg gggatgttcg aaggggaaat acttgtattc tataggtcat atcttgtttt
    10981 tattggcaca aatataatta cattagcttt ttgagggggc aataaacagt aaacacgatg
    11041 gtaataatgg taaaaaaaaa aacaagcagt tatttcggat atatgtcggc tactccttgc
    11101 gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg atgagaatgg
    11161 ccagaccatg atgaaataac ataaggtggt cccgtcggca agagacatcc acttaacgta
    11221 tgcttgcaat aagtgcgagt gaaaggaata gtattctgag tgtcgtattg agtctgagtg
    11281 agacagcgat atgattgttg attaaccctt agcatgtccg tggggtttga attaactcat
    11341 aatattaatt agacgaaatt atttttaaag ttttattttt aataatttgc gagtacgcaa
    11401 agcttcctag gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
    11461 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
    11521 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
    11581 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
    11641 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
    11701 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
    11761 cgcgccagcc gaactgttcg ccaagcttgg gctgcatcac gtaataagtg tgcgttgaat
    11821 ttattcgcaa aaacattgca tattttcggc aaagtaaaat tttgttgcat accttatcaa
    11881 aaaataagtg ctgcatactt tttagagaaa ccaaataatt ttttattgca tacccgtttt
    11941 taataaaata cattgcatac cctcttttaa taaaaaatat tgcatacttt gacgaaacaa
    12001 attttcgttg catacccaat aaaagattat tatattgcat acccgttttt aataaaatac
    12061 attgcatacc ctcttttaat aaaaaatatt gcatacgttg acgaaacaaa ttttcgttgc
    12121 atacccaata aaagattatt atattgcata ccttttcttg ccataccatt tagccgatca
    12181 attgtgctcg gcaaca
//
