LOCUS       sequence-114480-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 588, Emission: 618.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HcRed1-Nucleus-7
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      58..67
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             64..747
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     CDS             772..792
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             796..816
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             820..840
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cggtgggcnt tagcagagct ggtttgtgaa ccgtcagatc cgctagcgct accggtcgcc
       61 accatggtga gcggcctgct gaaggagagt atgcgcatca agatgtacat ggagggcacc
      121 gtgaacggcc actacttcaa gtgcgagggc gagggcgacg gcaacccctt cgccggcacc
      181 cagagcatga gaatccacgt gaccgagggc gcccccctgc ccttcgcctt cgacatcctg
      241 gccccctgct gcgagtacgg cagcaggacc ttcgtgcacc acaccgccga gatccccgac
      301 ttcttcaagc agagcttccc cgagggcttc acctgggaga gaaccaccac ctacgaggac
      361 ggcggcatcc tgaccgccca ccaggacacc agcctggagg gcaactgcct gatctacaag
      421 gtgaaggtgc acggcaccaa cttccccgcc gacggccccg tgatgaagaa caagagcggc
      481 ggctgggagc ccagcaccga ggtggtgtac cccgagaacg gcgtgctgtg cggccggaac
      541 gtgatggccc tgaaggtggg cgaccggcac ctgatctgcc accactacac cagctaccgg
      601 agcaagaagg ccgtgcgcgc cctgaccatg cccggcttcc acttcaccga catccggctc
      661 cagatgctgc ggaagaagaa ggacgagtac ttcgagctgt acgaggccag cgtggcccgg
      721 tacagcgacc tgcccgagaa ggccaactcc ggactcagat ctcgagctga tccaaaaaag
      781 aagagaaagg tagatccaaa aaagaagaga aaggtagatc caaaaaagaa gagaaaggta
      841 ggatccaccg
//
