LOCUS       sequence-114488-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase 
            activity; targeted to cytosolic face of lysosomal membrane..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Zhou X, Clister TL, Lowry PR, Seldin MM, Wong GW, Zhang J
  TITLE     Dynamic Visualization of mTORC1 Activity in Living Cells.
  JOURNAL   Cell Rep. 2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031.
  PUBMED    25772363
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031."
COMMENT     Sequence Label: pcDNA3-Lyso-TORCAR
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     96..117
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
       61 gccctcgccg gacacgctga acctgtggcc gtttacgtcg ccgtccagct cgaccaggat
      121 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atgggatccg caccaccgcc
      181 accaccgata gtctggtagc ctgcgtgact cctcttcctg ccgacgaggt aggcgatgag
      241 gacgatgagg accagccccg ccagggcacc acccacagcg atggggatca gcatgctgtt
      301 ctcgtccagc agacactcct ccacagagcc aaactggcca ccttccacct tgaaagcctg
      361 gacccacact ttgaatatat tgactgaaaa cgccttcgtg acacggacgt gctcctccgc
      421 gttgcacttg taggaattgc cgactgtggc ctgcagcgct cgcagggagc cgttggcagc
      481 tttaaaggca gggtctctgg cgtcaggaag aattgtattc aactggattc cttgtaggaa
      541 aaaccggcta gaacttgcat tcatcccgaa ctggaagagc aggacggtgg tgccctcgct
      601 gtgcagctcc agagtcacca ggtgggcgcc gcagctcccg ctggccgagg tcttgttggg
      661 gttgatgttg agaagccttg tcaccgtcgt gttgtccttc ctctcatagg tgaggttcag
      721 ctgcagcccc atgctggcca gcaggcaggt cccgttggtg ccgctcacgt tgtacttgtc
      781 cacaga
//
