LOCUS       sequence-114502-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pre-Assembled Ura3 integration vector to be used in the Dueber YTK 
            system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK096
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             688..699
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cgctgcggtt acgaactcca gcagaaccat atgatcgcgt ttctcgttcg gatctttaga
       61 cagaacgctt tgcgtgctca gatagtgatt gtctggcagc agaacaggac catcaccgat
      121 tggagtgttt tgctggtagt gatcagccag ctgcacgctg ccatcctcca cgttgtggcg
      181 aattttaaaa ttcgctttaa tgccattttt ttgtttatcg gcggtgatgt aaacattgtg
      241 gctgttaaaa ttgtattcca gcttatggcc caggatattg ccgtcctctt taaagtcaat
      301 gcctttcagc tcaatgcggt ttaccagggt atcgccttca aatttcactt ccgcacgcgt
      361 tttgtacgtg ccgtcatcct taaaggaaat cgtgcgttcc tgcacatagc cttccggcat
      421 ggcggacttg aagaagtcat gctgcttcat atggtccgga taacgagcaa agcactgaac
      481 accataagtc agcgtcgtta ccagagtcgg ccaaggaacc ggcagtttac cagtagtaca
      541 gatgaacttc agcgtcagtt taccattagt tgcgtcacct tcaccctcgc cacgcacgga
      601 aaacttatga ccgttgacat caccatccag ttccaccaga atagggacga caccagtgaa
      661 cagctcttcg cctttacgca tctagtattt ctcctctttc tctagtagct aagatccgaa
      721 cctgccgttt cttgagttgc cgcgatgtta agaaaacatt cataaattaa atgtgaattg
      781 ccgcacacat tattaaataa gatttacaaa atgttcaaaa tgacgcatga
//
