LOCUS       sequence-114517-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lysosome-targeted FRET biosensor for monitoring Akt activity; 
            targeted to cytosolic face of lysosomal membrane..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Zhou X, Clister TL, Lowry PR, Seldin MM, Wong GW, Zhang J
  TITLE     Dynamic Visualization of mTORC1 Activity in Living Cells.
  JOURNAL   Cell Rep. 2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031.
  PUBMED    25772363
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031."
COMMENT     Sequence Label: pcDNA3-Lyso-AktAR2
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     470..491
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(597..618)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tactactagg agaagacggt aaccttttat tgaatgacat ttccactaat gggacctggt
       61 taaatgggca aaaagtcgag aagaacagca atcagttact gtctcaaggt gatgaaataa
      121 ccgttggtgt aggcgtggaa tcagatattt tatctctggt cattttcata aacgacaaat
      181 ttaagcagtg cctcgagcag aacaaagttg atcgctctgc aggtaagcca ggcagcggcg
      241 agggcagcac caagggcctg gtcgaccctc gtccgcgctc gtgcacctgg ccggacccca
      301 ggccggagtt tggaggtacc ggcggcagcg agctcatggg cggcgtgcag ctcgccgacc
      361 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      421 tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      481 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggaggta
      541 ccggtggatc tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      601 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      661 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
      721 cctggcccac cctcgtgacc accctgggct acggccttca gtgcttcgcc cgctaccccg
      781 accacatgaa gcagcacgac ttcttcaagt ccgccatg
//
