LOCUS       sequence-114518-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Kraynov VS, Chamberlain C, Bokoch GM, Schwartz MA, Slabaugh S, Hahn 
            KM
  TITLE     Localized Rac activation dynamics visualized in living cells.
  JOURNAL   Science. 2000 Oct 13. 290(5490):333-7.
  PUBMED    11030651
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2000 Oct 13. 290(5490):333-7."
COMMENT     Sequence Label: pcDNA3-EGFP-Rac1(Q61L)
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(46..441,442..444,445..624))
                     /codon_start=1
                     /product="constitutively active mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (Q61L)
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGLEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     primer_bind     complement(673..694)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tttaggtgac actatagaat agggccctct agatgcatgc tcgagttaca acagcaggca
       61 ttttctcttc ctcttcttca cgggaggcgg gcagaggact gctcggatcg cttcgtcaaa
      121 cactgtcttg aggcctcgct gtgtgagcgc cgagcactcc aggtatttta cagcaccaat
      181 ctccttagcc atggctagac cctgcggata ggtgatggga gtcagcttct tctccttcag
      241 tttctcgatc gtgtctttat catccctaag atcaagttta gttcccacta ggatgatggg
      301 agtgttggga cagtggtgcc gcacctcagg ataccacttt gcacggacat tttcaaatga
      361 tgcaggactc acaagggaaa agcaaattaa gaacacatct gtttgcggat aggatagggg
      421 gcgtaatctg tcataatctt ctagtccagc tgtatcccat aagcccagat tcaccggttt
      481 tccatctacc ataacattgg cagaataatt gtcaaagaca gtagggatat attctccagg
      541 aaatgcattg gttgtgtaac tgatcagtag gcaagtttta cctacagctc cgtctcccac
      601 caccacacac ttgatggcct gcatgaattc cttgtacagc tcgtccatgc cgagagtgat
      661 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      721 gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
      781 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
      841 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt
      901 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      961 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
     1021 ggtcttgt
//
