LOCUS       sequence-114526-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT
            promotes cellular uptake of recombinant Cre into mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Peitz M, Pfannkuche K, Rajewsky K, Edenhofer F
  TITLE     Ability of the hydrophobic FGF and basic TAT peptides to promote 
            cellular uptake of recombinant Cre recombinase: a tool for efficient
            genetic engineering of mammalian genomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Apr 2. 99(7):4489-94.
  PUBMED    11904364
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Apr 2. 99(7):4489-94."
COMMENT     Sequence Label: pTriEx-HTNC
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(84..131)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     201..220
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    219..274
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     320..339
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(429..452)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 tgaacagagg tgcgtctggt gcaaactcct ttactttgaa caccagggaa acttcaagga
       61 gaatttcctc cgcatgcttt cagcaaaaaa cccctcaaga cccgtttaga ggccccaagg
      121 ggttatgcta gttacatatg ggcatatgtt gccaaactct aaaccaaata ctcattctga
      181 tgttttaaat gatttgccct cccatatgtc cttccgagtg agagacacaa aaaattccaa
      241 cacactattg caatgaaaat aaatttcctt tattagccag aagtcagatg ctcaaggggc
      301 ttcatgatgt ccccataatt tttggcagag ggaaaaagat cgatctcagt ggtatttgtg
      361 agccagggca ttggccacac cagccaccac cttctgatag gcagcctgca cctgaggtta
      421 atcacttagt gatggtgatg gtgatggtgg tgctcgagct aatcgccatc ttccagcagg
      481 cgcaccattg cccctgtttc actatccagg ttacggatat agttcatgac aatatttaca
      541 ttggtccagc caccagcttg catgatctcc ggtattgaaa ctccagcgcg ggccatatct
      601 cgcgcggctc cgacacgggc actgtgtcca gaccaggcca ggtatctctg accagagtca
      661 tccttagcgc cgtaaatcaa tcgatgagtt gcttcaaaa
//
