LOCUS       sequence-114534-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Kitay BM, McCormack R, Wang Y, Tsoulfas P, Zhai RG
  TITLE     Mislocalization of neuronal mitochondria reveals regulation of 
            Wallerian degeneration and NMNAT/WLDS-mediated axon protection 
            independent of axonal mitochondria.
  JOURNAL   Hum Mol Genet. 2013 Jan 24.
  PUBMED    23314018
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2013 Jan 24."
COMMENT     Sequence Label: pLV-mitoGFP
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        19..222
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     173..193
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     219..243
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             384..458
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             495..521
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     complement(552..580)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
       61 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      121 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      181 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
      241 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg cccgggcgta
      301 cgggatccca ccggtgctcg agatgcatac tagtggatcc cccgggctgc aggaattcga
      361 taattcgggt actccgtgcc atcatgtccg tcctgacgcc gctgctgctg cggggcttga
      421 caggctcggc ccggcggctc ccagtgccgc gcgccaagat ccattcgttg ccgccggagg
      481 ggaagcttat cgattatgat gttcctgatt atgcaagctt aatgagtaaa ggagaagaac
      541 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
      601 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
      661 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttctcttatg
      721 gtgttcaatg cttttcaaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg
      781 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
      841 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaa
//
