LOCUS       sequence-114536-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  generating transgenic TRIC flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pQUAST-frt-stop-frt-p65AD::CaM
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..59)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(124..171)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 ggcaggtact ggaactccat cggcgtggat cccaattcga cctttctctt cttttttgga
       61 ggctcgggaa ttaattccgc tttatccatg ttgagatctg gtacccagct tcaaaagcgc
      121 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caagatcccc
      181 cggatcctcg catggttttg cctaattgtt tcagatttat ggggcttaca tacatacata
      241 attactttat acaacgaaaa ttactaaata gaatcgttcc tgtaagcagg actgacacat
      301 ttgcttgcgt tgattgctat tatatcgacc taccatagtc acataaatat gtacatatat
      361 aaatatgtat gtatgtacaa tcgcatgctt gacggcagtt aagaaatgaa gtcaccattt
      421 cggtgagctg gcaacgatta tgctgtggcc ccgaaaggaa tgactcatcg tcccagagaa
      481 tcgataccaa cggtggaaag ccaagtgcat gccacactgc gtgcacagcc tgctgctaca
      541 cttgccacaa acagcaggcg gaaaagcgca gcagcggccc gaatcgggga aaatagaaag
      601 cgctaattat agacgaagtg aacggcagct ctgctttccc gcagtcgaaa ctccgtgcaa
      661 tgaccccgtt ctttcaccac gaaatgaatg atcgctggga gaaagaaaag gcaaagaaaa
      721 acatagtggg gttttcttat ttctgacaac actgaatctg gccgatttta ttcttgtgta
      781 cacaacttat cgccgagtta acttggcttt tctgctatac gtgtctt
//
