LOCUS       sequence-114540-         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Chen Y, Partow S, Scalcinati G, Siewers V, Nielsen J
  TITLE     Enhancing the copy number of episomal plasmids in Saccharomyces 
            cerevisiae for improved protein production.
  JOURNAL   FEMS Yeast Res. 2012 Aug;12(5):598-607. doi: 
            10.1111/j.1567-1364.2012.00809.x. Epub 2012 Apr 25.
  PUBMED    22487308
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1567-1364.2012.00809.x"; journalName: "FEMS Yeast Res"; 
            date: "2012-08"; volume: "12"; issue: "5"; pages: "598-607"
COMMENT     Sequence Label: pSP-GM2
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(170..193)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tcataaatca taagaaattc gcttatttag aagtgtcaac aacgtatcta ccaacgattt
       61 gacccttttc catcttttcg taaatttctg gcaaggtaga caagccgaca accttgattg
      121 gagacttgac caaacctctg gcgaagaatt gttaattaag agctcagatc ttatcgtcgt
      181 catccttgta atccatcgat actagtgcgg ccgcttgttt tatatttgtt gtaaaaagta
      241 gataattact tccttgatga tctgtaaaaa agagaaaaag aaagcatcta agaacttgaa
      301 aaactacgaa ttagaaaaga ccaaatatgt atttcttgca ttgaccaatt tatgcaagtt
      361 tatatatatg taaatgtaag tttcacgagg ttctactaaa ctaaaccacc cccttggtta
      421 gaagaaaaga gtgtgtgaga acaggctgtt gttgtcacac gattcggaca attctgtttg
      481 aaagagagag agtaacagta cgatcgaacg aactttgctc tggagatcac agtgggcatc
      541 atagcatgtg gtactaaacc ctttcccgcc attccagaac cttcgattgc ttgttacaaa
      601 acctgtgagc cgtcgctagg accttgttgt gtgacgaaat tggaagctgc aatcaatagg
      661 aagacaggaa gtcgagcgtg tctgggtttt ttcagttttg ttctttttgc
//
