LOCUS       sequence-114546-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Partow S, Siewers V, Bjorn S, Nielsen J, Maury J
  TITLE     Characterization of different promoters for designing a new 
            expression vector in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2010 Nov;27(11):955-64. doi: 10.1002/yea.1806.
  PUBMED    20625983
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea"; 
            journalName: "Yeast"; date: "2010-11"; volume: "27"; issue: "11"; 
            pages: "955-64"
COMMENT     Sequence Label: pSP-G1
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(33..222)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(252..281)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(298..317)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(299..317)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagctgg atcttcgagc gtcccaaaac cttctcaagc
       61 aaggttttca gtataatgtt acatgcgtac acgcgtctgt acagaaaaaa aagaaaaatt
      121 tgaaatataa ataacgttct taatactaac ataactataa aaaaataaat agggacctag
      181 acttcaggtt gtctaactcc ttccttttcg gttagagcgg atcttagcta gccgcggtac
      241 caagcttact cgaggtcttc ttcggaaatc aacttctgtt ccatgtcgac gcccgggccc
      301 tatagtgagt cgtattacgg atccttgttt tatatttgtt gtaaaaagta gataattact
      361 tccttgatga tctgtaaaaa agagaaaaag aaagcatcta agaacttgaa aaactacgaa
      421 ttagaaaaga ccaaatatgt atttcttgca ttgaccaatt tatgcaagtt tatatatatg
      481 taaatgtaag tttcacgagg ttctactaaa ctaaaccacc cccttggtta gaagaaaaga
      541 gtgtgtgaga acaggctgtt gttgtcacac gattcggaca attctgtttg aaagagagag
      601 agtaacagta cgatcgaacg aactttgctc tggagatcac agtgggcatc atagcatgtg
      661 gtactaaacc ctttcccgcc attccagaac cttcgattgc ttgttac
//
