LOCUS       sequence-114551-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Chen Y, Daviet L, Schalk M, Siewers V, Nielsen J
  TITLE     Establishing a platform cell factory through engineering of yeast 
            acetyl-CoA metabolism.
  JOURNAL   Metab Eng. 2013 Jan;15:48-54. doi: 10.1016/j.ymben.2012.11.002. Epub
            2012 Nov 17.
  PUBMED    23164578
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2013-01"; volume: "15:48-54. doi"; pages: " 
            10.1016/j.ymben.2012.11.002. Epub 2012 Nov 17"
COMMENT     Sequence Label: pIYC04
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(178..365)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(512..531)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     631..653
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tatacctgtg tggacgttaa tcacttgcga ttgtgtggcc tgttctgcta ctgcttctgc
       61 ctctttttct gggaagatcg agtgctctat cgctagggga ccacccttta aagagatcgc
      121 aatctgaatc ttggtttcat ttgtaatacg ctttactagg gctttctgct ctgtcatctt
      181 tgccttcgtt tatcttgcct gctcattttt tagtatattc ttcgaagaaa tcacattact
      241 ttatataatg tataattcat tatgtgataa tgccaatcgc taagaaaaaa aaagagtcat
      301 ccgctaggtg gaaaaaaaaa aatgaaaatc attaccgagg cataaaaaaa tatagagtgt
      361 actagaggag gccaagagta atagaaaaag aaaattgcgg gaaaggactg tgttatgact
      421 tccctgacta atgccgtgtt caaacgatac ctggcagtga ctcctagcgc tcaccaagct
      481 cttaaaacgg gaatttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
      541 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
      601 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
      661 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgccta
//
