LOCUS       sequence-114554-         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Chen Y, Daviet L, Schalk M, Siewers V, Nielsen J
  TITLE     Establishing a platform cell factory through engineering of yeast 
            acetyl-CoA metabolism.
  JOURNAL   Metab Eng. 2013 Jan;15:48-54. doi: 10.1016/j.ymben.2012.11.002. Epub
            2012 Nov 17.
  PUBMED    23164578
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2013-01"; volume: "15:48-54. doi"; pages: " 
            10.1016/j.ymben.2012.11.002. Epub 2012 Nov 17"
COMMENT     Sequence Label: pIYC04
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(60..249)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(279..308)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(325..344)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(326..344)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagctgg ataaacgccg gcgaaacgat ccggaggatc
       61 ttcgagcgtc ccaaaacctt ctcaagcaag gttttcagta taatgttaca tgcgtacacg
      121 cgtctgtaca gaaaaaaaag aaaaatttga aatataaata acgttcttaa tactaacata
      181 actataaaaa aataaatagg gacctagact tcaggttgtc taactccttc cttttcggtt
      241 agagcggatc ttagctagcc gcggtaccaa gcttactcga ggtcttcttc ggaaatcaac
      301 ttctgttcca tgtcgacgcc cgggccctat agtgagtcgt attacggatc cttgttttat
      361 atttgttgta aaaagtagat aattacttcc ttgatgatct gtaaaaaaga gaaaaagaaa
      421 gcatctaaga acttgaaaaa ctacgaatta gaaaagacca aatatgtatt tcttgcattg
      481 accaatttat gcaagtttat atatatgtaa atgtaagttt cacgaggttc tactaaacta
      541 aaccaccccc ttggttagaa gaaaagagtg tgtgagaaca ggctgttgtt gtcacacgat
      601 tcggacaatt ctgtttgaaa gagagagagt aacagtacga tcgaacgaac tttgctctgg
      661 agatcacagt gggcatcata gcatgtggta ct
//
