LOCUS       sequence-114560-         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Chen Y, Partow S, Scalcinati G, Siewers V, Nielsen J
  TITLE     Enhancing the copy number of episomal plasmids in Saccharomyces 
            cerevisiae for improved protein production.
  JOURNAL   FEMS Yeast Res. 2012 Aug;12(5):598-607. doi: 
            10.1111/j.1567-1364.2012.00809.x. Epub 2012 Apr 25.
  PUBMED    22487308
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1567-1364.2012.00809.x"; journalName: "FEMS Yeast Res"; 
            date: "2012-08"; volume: "12"; issue: "5"; pages: "598-607"
COMMENT     Sequence Label: pSP-GM1
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(60..249)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(279..308)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(325..344)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(326..344)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagctgg ataaacgccg gcgaaacgat ccggaggatc
       61 ttcgagcgtc ccaaaacctt ctcaagcaag gttttcagta taatgttaca tgcgtacacg
      121 cgtctgtaca gaaaaaaaag aaaaatttga aatataaata acgttcttaa tactaacata
      181 actataaaaa aataaatagg gacctagact tcaggttgtc taactccttc cttttcggtt
      241 agagcggatc ttagctagcc gcggtaccaa gcttactcga ggtcttcttc ggaaatcaac
      301 ttctgttcca tgtcgacgcc cgggccctat agtgagtcgt attacggatc cttgttttat
      361 atttgttgta aaaagtagat aattacttcc ttgatgatct gtaaaaaaga gaaaaagaaa
      421 gcatctaaga acttgaaaaa ctacgaatta gaaaagacca aatatgtatt tcttgcattg
      481 accaatttat gcaagtttat atatatgtaa atgtaagttt cacgaggttc tactaaacta
      541 aaccaccccc ttggttagaa gaaaagagtg tgtgagaaca ggctgttgtt gtcacacgat
      601 tcggacaatt ctgtttgaaa gagagagagt aacagtacga tcgaacgaac tttgctctgg
      661 agatcacagt
//
