LOCUS       sequence-114561-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Chen Y, Partow S, Scalcinati G, Siewers V, Nielsen J
  TITLE     Enhancing the copy number of episomal plasmids in Saccharomyces 
            cerevisiae for improved protein production.
  JOURNAL   FEMS Yeast Res. 2012 Aug;12(5):598-607. doi: 
            10.1111/j.1567-1364.2012.00809.x. Epub 2012 Apr 25.
  PUBMED    22487308
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1567-1364.2012.00809.x"; journalName: "FEMS Yeast Res"; 
            date: "2012-08"; volume: "12"; issue: "5"; pages: "598-607"
COMMENT     Sequence Label: pSP-GM1
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(170..193)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(227..633)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 tcataaatca taagaaattc gcttatttag aagtgtcaac aacgtatcta ccaacgattt
       61 gacccttttc catcttttcg taaatttctg gcaaggtaga caagccgaca accttgattg
      121 gagacttgac caaacctctg gcgaagaatt gttaattaag agctcagatc ttatcgtcgt
      181 catccttgta atccatcgat actagtgcgg ccgcttgtaa ttaaaactta gattagattg
      241 ctatgctttc tttctaatga gcaagaagta aaaaaagttg taatagaaca agaaaaatga
      301 aactgaaact tgagaaattg aagaccgttt attaacttaa atatcaatgg gaggtcatcg
      361 aaagagaaaa aaatcaaaaa aaaaaatttt caagaaaaag aaacgtgata aaaattttta
      421 ttgccttttt cgacgaagaa aaagaaacga ggcggtctct tttttctttt ccaaaccttt
      481 agtacgggta attaacgaca ccctagagga agaaagaggg gaaatttagt atgctgtgct
      541 tgggtgtttt gaagtggtac ggcgatgcgc ggagtccgag aaaatctgga agagtaaaaa
      601 aggagtagaa acattttgaa gctatggtgt gtgcggccgg cctggaagta ccttcaaaga
      661 atggggtctt atcttgtttt gcaagtacca ctgagcagga taataataga aatgataata
      721 tactatagta gagataacgt cgatgac
//
