LOCUS       sequence-114563-         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Partow S, Siewers V, Bjorn S, Nielsen J, Maury J
  TITLE     Characterization of different promoters for designing a new 
            expression vector in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2010 Nov;27(11):955-64. doi: 10.1002/yea.1806.
  PUBMED    20625983
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea"; 
            journalName: "Yeast"; date: "2010-11"; volume: "27"; issue: "11"; 
            pages: "955-64"
COMMENT     Sequence Label: pSP-G2
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(170..193)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tcataaatca taagaaattc gcttatttag aagtgtcaac aacgtatcta ccaacgattt
       61 gacccttttc catcttttcg taaatttctg gcaaggtaga caagccgaca accttgattg
      121 gagacttgac caaacctctg gcgaagaatt gttaattaag agctcagatc ttatcgtcgt
      181 catccttgta atccatcgat actagtgcgg ccgcttgttt tatatttgtt gtaaaaagta
      241 gataattact tccttgatga tctgtaaaaa agagaaaaag aaagcatcta agaacttgaa
      301 aaactacgaa ttagaaaaga ccaaatatgt atttcttgca ttgaccaatt tatgcaagtt
      361 tatatatatg taaatgtaag tttcacgagg ttctactaaa ctaaaccacc cccttggtta
      421 gaagaaaaga gtgtgtgaga acaggctgtt gttgtcacac gattcggaca attctgtttg
      481 aaagagagag agtaacagta cgatcgaacg aactttgctc tggagatcac agtgggcatc
      541 atagcatgtg gtactaaacc ctttcccgcc attccagaac cttcgattgc ttgttacaaa
      601 acctgtgagc cgtcgctagg accttgttgt gtgacg
//
