LOCUS       sequence-114564-         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Partow S, Siewers V, Bjorn S, Nielsen J, Maury J
  TITLE     Characterization of different promoters for designing a new 
            expression vector in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2010 Nov;27(11):955-64. doi: 10.1002/yea.1806.
  PUBMED    20625983
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea"; 
            journalName: "Yeast"; date: "2010-11"; volume: "27"; issue: "11"; 
            pages: "955-64"
COMMENT     Sequence Label: pSP-G2
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(193..222)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(239..258)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(240..258)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caaggttttc agtataatgt tacatgcgta cacgcgtctg tacagaaaaa aaagaaaaat
       61 ttgaaatata aataacgttc ttaatactaa cataactata aaaaaataaa tagggaccta
      121 gacttcaggt tgtctaactc cttccttttc ggttagagcg gatcttagct agccgcggta
      181 ccaagcttac tcgaggtctt cttcggaaat caacttctgt tccatgtcga cgcccgggcc
      241 ctatagtgag tcgtattacg gatccttgta attaaaactt agattagatt gctatgcttt
      301 ctttctaatg agcaagaagt aaaaaaagtt gtaatagaac aagaaaaatg aaactgaaac
      361 ttgagaaatt gaagaccgtt tattaactta aatatcaatg ggaggtcatc gaaagagaaa
      421 aaaatcaaaa aaaaaaattt tcaagaaaaa gaaacgtgat aaaaattttt attgcctttt
      481 tcgacgaaga aaaagaaacg aggcggtctc ttttttcttt tccaaacctt tagtacgggt
      541 aattaacgac accctagagg aagaaagagg ggaaatttag tatgctgtgc ttgggtgttt
      601 tgaagtggta cggcgatgcg cggagtccga g
//
