LOCUS       sequence-114568-       13599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic 
            configuration with optimized promoters (E12).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13599)
  AUTHORS   Jones JA, Vernacchio VR, Lachance DM, Lebovich M, Fu L, Shirke AN, 
            Schultz VL, Cress B, Linhardt RJ, Koffas MA
  TITLE     ePathOptimize: A Combinatorial Approach for Transcriptional 
            Balancing of Metabolic Pathways.
  JOURNAL   Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
  PUBMED    26062452
REFERENCE   2  (bases 1 to 13599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-11"; volume: "5:11301. doi"; pages: " 
            10.1038/srep11301"
COMMENT     Sequence Label: pETM6-E12-vioABECD
FEATURES             Location/Qualifiers
     source          1..13599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    6..29
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             44..66
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1376..1420
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1473..1520
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    1573..1597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1612..1634
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4684..4728
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      4781..4828
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    4881..4905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4920..4942
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5568..5612
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      5665..5712
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    5766..5789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5804..5826
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7136..7180
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      7233..7280
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    7333..7357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7372..7394
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             8536..8580
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      8633..8680
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      8724..9179
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     9021..9042
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(9294..10085,10086..10154))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9917..9936
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10155..10247)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10328..10916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10817..10836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11070..11087
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11102..11244
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11170..11189)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11330..11352
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(11346..11537)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(12109..13191)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     13153..13172
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(13192..13269)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     13475..13494
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cggaagaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga
       61 aggagatata catatgttaa atttagagca ttcagtttca attgtcggag ctggaatttc
      121 tgggatagtg tgtgcaatta cattagctcg ttcaagggca ttagaaggtt acgcaataga
      181 tgtctatgaa caaagccccc gcgttggtgg acggatccaa acaaacttgt tgagcaatca
      241 ttacatcgat ttaggtgccg ctcgtttttg ttcacaattg catggtaatg tatatgatct
      301 agtcaagagg tttgaactcg aatttgaacc gtttcctttt actcaactta gatttactca
      361 gccaattcaa aaagaactca agactatttt gtctaatcta aaaccgttaa tcgatgaaca
      421 taaagaagat tcttttgtcg aatttgttac gagctatttg ggtaaagaaa aagcaaaagc
      481 agtaatctta gcactgggct acgactcact ctctatgcca ataatttctc cacatattgc
      541 ttacgatatc attgaaaaac acccagaaac tcaatgtttc actgaaaatt gtgggaatga
      601 atggtttagt ttgaaagagg gattggcgtc gttgatcaag gcgctatatg aagaggcgtt
      661 aaaactcggg attaagtttc attttgaatc tacattagtt caaataagca atcgaggtaa
      721 cgcccacagc ttggtgattg ccgataatca aaatcaacaa aaagagatca gtaaagggct
      781 taagatcatt actttgcctc catctcgaat ggaaaaatta gataagcaat ttcctaaacg
      841 ttggagcaac tacaattatg gttctagccc cttgtttaaa ggatttttct tttactccga
      901 accgtggtgg cgagagttag ggttgacgga tcacgtcgta attacccaaa atccgatccg
      961 taaattgtat ttcaaagatg aaaaatacgc gtttttctat actgatagtg acaacgcgtt
     1021 atattggcga gatgtgcttt ctttaggcga agatcattac cttacaacgg ttaaaaagct
     1081 tatcgcggag gcactaaact tacctcaaga tgtcattcca aatccagagt tacacaaaaa
     1141 caaattttgg cttcatggtg tcgaattttc aaaagaatgt tgccctaaac atccatttta
     1201 tcaaacactt tctgatgaca gcgttatctc tttgtctgac gcctatacac accattgtgg
     1261 atggatagaa gggggcatct tagccggcaa gtttacagcg gaatcgatca tcagccagtt
     1321 gcctacacat cgtgaaactg tgaaagaagt agtatacctt tgactcgagt ctggtaaaga
     1381 aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagtc gcagcttaat
     1441 taacctaaac tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
     1501 gggtcttgag gggttttttg ctaggatcga gatcgatctc gatcccgcga aattaatacg
     1561 actcactatt tcggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa
     1621 ctttaagaag gagatataca tatgagtgtt ttagattttc ctcgaataca ttttaagggt
     1681 atggctcggg ttaatatacc aaccgcaaac cgaaataccc aaaacacatt ggatacgaca
     1741 acaaataccg tgtttcaatt cggccagccg gttgacattg acttgccgcc ggcacgtttc
     1801 cacgaatacc tccactcact tggtccaaaa gttaatcgct ttggtaaaaa agatgaaaat
     1861 ggagaattca gtgtagcgtg tggctacaac atgcttggta ataatcattt ttcatggcac
     1921 gacgctaaag ttacggcttt gcaattgcgc ccgggtgatt gtgttgttca agatccactc
     1981 atagggtgtt ctgttaattt atggggacac tacaatgagt accttagaac ctcattcaat
     2041 cgtgctcgct gggttgataa tgacccgacc cgaagtgact cggcactcat ctatgctgga
     2101 caaattacat taactgcaaa tggcagtgag gcaagtgaaa agcatgtgat gtctgctgac
     2161 attgattgta cacatggagt gagatggata aatcctagct ttattaatag cctcgacgaa
     2221 catgcgtttg ctgaagatat tgcagcagcc cgtttgtttc agtttgctgt caataagaac
     2281 agcaaggatt tcttattgaa tcaattgggc ttgcagtcag aatttattga acaattacga
     2341 atagcactag aacaagaaga tgttaagggc ttagttgttc aatattgtgt aagcaatcta
     2401 tcgcctcctg tagcgcccaa tatgccggtc ttttgtgatt tacatggctg catagggctt
     2461 tggcgagaaa gtgaaatggc cacttgtcct gcaggtcgga ttttacagcc agaatgccca
     2521 cagcaatttg caccgattac attaaaacag gcagggcaat ggttgagtat aaatatgccg
     2581 attagtatcg gtcatactca tcacttagat ccattacctg tattatctgg aatgcctcct
     2641 cgattagcag caaaaacgcc cttgggtgtc ttgcaactca agacggccag tgatgagtta
     2701 atcgccgaga tcccagaggt gctttataac gcgtgtttgg atacaggagg gatcatcgat
     2761 gtgacaatac aaacccatac agacggactt gaattacagt cattaaagct ggtaagccag
     2821 tctcattcgt ggcatgaaat cgattggcaa gttcaagccg aacaacacat catatatatg
     2881 gaatctccaa acctaatcga gttgcaaaaa agcaagcaat atattgatgt atatagctat
     2941 tttcgtggaa ttcttaagcc aatcaacggt ttaacaagtt ataacgcggg accacaacga
     3001 gtgcaatgtg acgagttcat tgaaacgaat gaacaaggac gtggtttgtt tctagttgaa
     3061 gcgttaacac cagggagtgg tgaagttttt ctaggagaac aatatagtcg tgtccagatc
     3121 cgtgtattaa gtaatgactg ggcgttactt aatgtccctg atgaacaggt tgactatgac
     3181 tttctttata aaaatgtcat ggcctactat gagtttgttt atccgttcat ggcagacaaa
     3241 gtgtttagca tggctgataa atgtaaatgc gaaacgtatg cacgcttaat gtggcaaatg
     3301 tgcgatccca aaaataaaga gaaaagctac tacatgccta gtacgcggga gatgtcacaa
     3361 gcacaatcgt ttctattttt gaaatatttg agtaacgtag aaaagtcggc aatccccaag
     3421 gttttgcctt attgcaatac agacgttacg gttacaggca aaataaaaaa ccgtacacaa
     3481 ttaattcggg cattaaaaga ctctgtcgac ctagagttgt cgattatgct gcagtatttg
     3541 tatagcgcct attcactacc aaattacgcc gccggagagg cgttggtaaa cgcaaaaaga
     3601 tggactcaag aacaacttga attagtgaat ggtggcgcag ataggcggca caacagcggt
     3661 tggcgaggtg ctattcttga agttgctcac gaagagatga tccattattt ggtcgtcaat
     3721 aatttgctta tgtccttagg cgaacctttt tactgtggca acccggtact tggccaacaa
     3781 gcgaacgaaa aatttggact cgatacggat ttttcttacg agccctttgg cttacagtcc
     3841 atcacgaaat ttgtacgctt tgagtggcca cgctttattc cgaccatagg tctttcaatt
     3901 gctgatttct atgccgacat taagcgtgcc tttgagcaat taccagacct ctatcctaaa
     3961 gatgcgaagg gttcgggggg agagcatcac ttgtttttga atgaagtgat taaccgtcaa
     4021 tacccggcat accaattcga agtgtataac cgacagactg ctttgtttgc aattgaattt
     4081 gttacagagc aaggcgaagg tgtgactgca cagtcagaac aattcgaatt aggtcatttc
     4141 caccgcttaa gacagatagc catggcatta actaatcaag aacagccttt tgaaccggcg
     4201 tatcccgtgc ttaaaaaccc tgtttttaaa cctagagcgg gatgtaattt agtcaccgac
     4261 ccagaatcac aagccttaat ggcgttttat caacagtgtc atgaattgat gtttcaaatc
     4321 atgatgcatc atttttcgca acgacctctc atcagcatgc ggcgctcgcg attgatgaat
     4381 gccgccatcg acctgatgac aggaatatta cgtccattgt ctgtgaaatt aatgacgatg
     4441 ccgtctggga ctaaaggtcg aaatgccggt ccgccagtgc cagagtcggt cagttctttc
     4501 gtcgataagg attatgctgg cggttgccaa caattgtcat ggcgctgcgc acagttggcg
     4561 gcagcggcaa aagcgttaaa cctaaatgta actacacaaa tcgaattatt ggagtttttc
     4621 caacaacaac tggctgacct cgcagccgga actctttcaa aggaaggtta gctcgagtct
     4681 ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc tactagtcgc
     4741 agcttaatta acctaaactg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     4801 ctctaaacgg gtcttgaggg gttttttgct aggatcgaga tcgatctcga tcccgcgaaa
     4861 ttaatacgac tcactatttc ggaattgtga gcggataaca attcccctct agaaataatt
     4921 ttgtttaact ttaagaagga gatatacata tggaattacg taaagtagat agagttccgc
     4981 caatgttacc tgagtgttgg agcagtagtt atatttctta ttggcttcct atgaagccag
     5041 aagacgatat tacttctggg tattgttggt tcgattatgc tcaagaggtt tgtcgtatag
     5101 acggtttgtt taacccttgg agtgaaacaa aaatgggaca ccgtttgtgg atgtccgaaa
     5161 ttatgtatcc acgcagttat gaatcgttta aatctaaaat tgcctatgca cgaacacatc
     5221 gggataaacc ttcagcgttt aaagaaacgg tgttaaatga tgaggtagac ccatgccatg
     5281 agcttatttt aacgcgtgat gtgttggtaa aaaaccacgc taagtatgca ggtgaacaca
     5341 ccattttggg ttataaagcg gatgcgtggt gctttgaacg cccaaatgac aaaggtcctg
     5401 caacgtattt ttttaaagaa gggacagatc agttgctccg aatggtcacg ggtgatccga
     5461 acgttcacgc ctctgtaaga gactttccta attttaatac gctgccgata cctaacacga
     5521 tttttgatgt aacaagtctc tctcatagga attgactcga gtctggtaaa gaaaccgctg
     5581 ctgcgaaatt tgaacgccag cacatggact cgtctactag tcgcagctta attaacctaa
     5641 actgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     5701 aggggttttt tgctaggatc gagatcgatc tcgatcccgc gaaattaata cgactcacta
     5761 cggaagaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga
     5821 aggagatata catatgagta aaataattat tgttggtggt gggttggcag gcagcctgac
     5881 ggcaatctat ctagcgaaac aagggcacga agttcacatt atagaaaaac gtggaaatcc
     5941 tttagaaagt caggcggatt atattgatca agttagcgcg cgtgcgattg gtgtcagtat
     6001 gacagtacga ggaattgaag ccgttttagc agccggtatt cccttggacg aattgcaggc
     6061 gtgcggcgtt gaagtttcag gtatggcgtt ttttatcaaa ggtcaaccta aaatacggga
     6121 tttaccaccg ttagatacgt taaaaccgtt gtcgttaagc cgaagcatgt ttcaggtatt
     6181 gcttaacaag tacgctcata aagcaaaagt acactatcac tatctacaac gctgtgtcga
     6241 cgttgatatt gaaagagcgt cagtgattac acaaaaccag tatggcgaat ttgttaacca
     6301 ccaaggtgat ttgctgattg ctgcggatgg cgcccgttca gttgtccgag acgcatttca
     6361 aactcatagt cgtcgttttg agtttcatca atcctttttt aagcatggtt ataaaacact
     6421 cgtgatccca gatgccacag aagtcggtct tcgcaaagac attttatatt tctttggtat
     6481 ggattctgga ggggaatttg caggtcgtgc ggcaactatc cctgatggca gtatcagttt
     6541 tgctgtgtgt ttaccttata ccggaaaagt aagtttacac tctgaagaca cgcaagcaat
     6601 gaaggatttc tttgaacgct attacgccat gatacctaag cacattcgag agtcgatgta
     6661 ccaacaattt atggaaaagc caagtaatga cttaattaat gttcgatcgt caacctttca
     6721 ttataaaaac agagcattgt tgttagggga tgcggcccat gctaccgcac cttttttagg
     6781 acagggaatg aatatggctt tagaagacgc ctttgtcctt gatagattgt ttgccgagtt
     6841 tgaaggaaac ttaaccgaaa tcttgcctcg ttttactgac ctacgcaaaa aagaagctga
     6901 tgcgatgcaa gatatggcgc gggcaaacta tgatgtcttg tcttgctcaa atccagtctt
     6961 ttttatgcgt gcgaaatttg cccgttatat gacaaaacaa ttcccagatc gctatccacc
     7021 cgatatggct gaaaaattat actttacgtc tatggcgtat agcgagcttc gggatgttca
     7081 acaaaaacag aatgtttggt acaaaatagg gagaatgaat taactcgagt ctggtaaaga
     7141 aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagtc gcagcttaat
     7201 taacctaaac tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
     7261 gggtcttgag gggttttttg ctaggatcga gatcgatctc gatcccgcga aattaatacg
     7321 actcactatt tcggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa
     7381 ctttaagaag gagatataca tatgaacatt ttagtgatcg gggcaggtcc tgccggatta
     7441 atgtttgcta gtcaaattaa aaaactgaat ccagattggg atatttgtat taaagaaaag
     7501 aataatcaag acgaaaccgt aggttggggc gtagtgctgc cagggcgcgc gccacatcat
     7561 cccgccaatc cactttcgta tcttcctaat tatgaatcct tggatgcaca atatttagat
     7621 gcctttaaaa tgactcacca caatgaaagt gcgttaaaaa gtacagggct aaccttatgc
     7681 ggcgctgagc gaaaatcgat gattaaggct atgcgagatt tgtgtattcg tttgggtatc
     7741 catattgatt atgagcaacc agttgaaaat cttgaattag ctagcactca atatgacttg
     7801 gttgtggtat caaacgggat aaaccacgcc aaccaattct atcgtgaagc gctaaaaccg
     7861 aacattgaat atggcacgaa tcgctacatg tggtatggca cgcctaatct ttttaatgaa
     7921 atgaacctga tttttaaagc gcattctaaa ggtgtttttg tcgctcatgc ctataaatat
     7981 tcgagtgaca tgagtacttt tgttgttgaa tgtagcgaag agacgtatct aaagtcaggt
     8041 ctagacgaaa tgtctgcaga ccaagcagaa gcgtttattg catccgtatt tgaatctgat
     8101 ttgaacggaa agcctgttat tgctcaatca ggattgaaat ggcgtaattt catgacgtta
     8161 agtcatgaaa aggcctatga tggaaatatt gtgttattgg gcgatgcgtt gcaatcgggt
     8221 catttttcaa tcgggcatgg cacgaccatg gcagtggttg gcgcacagtt actgattaaa
     8281 tctgtccatc aacatgcaaa taacgtcatt gctgtggtag agcactttaa tacgcgagtg
     8341 atgcctgtca tgaaattgtt tgatcaacat gcacaaacaa gtcgcctttg gtttgaaagt
     8401 gtaggagagc taatgcatct aagtacttct caaatcgctc aaagttttac tgaacgtcgt
     8461 gagcagctgc cttctttacc agaagcgcta gcaaaggcat taggttatgc gctgcaacgt
     8521 taactcgagt ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg
     8581 tctactagtc gcagcttaat taacctaaac tgctgccacc gctgagcaat aactagcata
     8641 accccttggg gcctctaaac gggtcttgag gggttttttg ctagcgaaag gaggagtcga
     8701 ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     8761 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     8821 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     8881 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     8941 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     9001 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     9061 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     9121 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc
     9181 tggcggcacg atggcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9241 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9301 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9361 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9421 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9481 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9541 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9601 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9661 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9721 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9781 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9841 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9901 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9961 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10021 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10081 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10141 tgttgaatac tcatactctt cctttttcaa tcatgattga agcatttatc agggttattg
    10201 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gtcatgacca
    10261 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    10321 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    10381 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    10441 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
    10501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    10561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    10621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    10681 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    10741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    10801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    10861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    10921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    11041 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    11101 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg
    11161 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
    11221 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
    11281 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
    11341 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
    11401 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
    11461 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
    11521 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
    11581 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
    11641 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
    11701 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
    11761 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
    11821 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
    11881 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
    11941 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgctagtc
    12001 atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga
    12061 gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
    12121 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    12181 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
    12241 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
    12301 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
    12361 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
    12421 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
    12481 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
    12541 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
    12601 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
    12661 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
    12721 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
    12781 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
    12841 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
    12901 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
    12961 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
    13021 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
    13081 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
    13141 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
    13201 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
    13261 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
    13321 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
    13381 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
    13441 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
    13501 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag cctaggatcg
    13561 agatcgatct cgatcccgcg aaattaatac gactcacta
//
